外用红油膏对大鼠体内汞蓄积及肝肾功能的影响

葛巍; 廖武; 张梦婕; 杨苏琴

文章摘要

葛巍，廖武，张梦婕，杨苏琴.外用红油膏对大鼠体内汞蓄积及肝肾功能的影响[J].实用中西医结合临床,2022,22(2):120-123

外用红油膏对大鼠体内汞蓄积及肝肾功能的影响

Influence of Topical Red Oil Ointment on Mercury Accumulation and Liver and Kidney Function in Rats

DOI：

中文关键词: 汞蓄积红油膏肝肾功能

英文关键词: Mercury accumulation Red oil ointment Liver and kidney function

基金项目:江西省中医药管理局科技计划项目（编号：2019A281）

作者	单位
葛巍，廖武，张梦婕，杨苏琴	江西中医药大学附属医院

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中文摘要:

目的：研究不同剂量的红油膏外用30 d对大鼠体内汞蓄积及肝肾功能的影响。方法：将40只大鼠随机分成正常组与红油膏低、中、高剂量组，每组10只。各组大鼠背部造成面积为3 cm×3 cm的全层皮肤缺损创面，除正常组外，红油膏低、中、高剂量组分别予以4.744 mg、94.875 mg、189.750 mg红油膏纱条外敷。持续给药30 d后检测四组大鼠尿液、血液、肝脏及肾脏的汞含量，肝肾功能指标血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、尿素氮（BUN）、肌酐（CREA）。结果：红油膏各剂量组的血液和尿液中汞含量均明显高于正常组（P＜0.05，P＜0.01）；红油膏中、高剂量组的肝脏与肾脏中汞含量均明显高于正常组（P＜0.01），其中尿液、肾脏中汞含量呈剂量依赖性（P＜0.01）；相比正常组，红油膏高剂量组中AST含量明显升高（P＜0.05），红油膏中、高剂量组中BUN与CREA含量均明显升高（P＜0.05，P＜0.01）。结论：红油膏长期、超量给药可造成大鼠体内汞蓄积及肝肾毒性，但日均剂量4.744 mg（相当于人体临床日均剂量230 mg）且持续30 d外用对肝肾汞蓄积及肝肾功能影响较小，可为红油膏临床安全性提供数据参考。

英文摘要:

Objective: To study the influence of different doses of red oil ointment on mercury accumulation and liver and kidney function in rats after external used 30 days. Methods: 40 rats were randomly divided into normal group, low-dose group of red oil ointment, medium-dose group of red oil ointment and high-dose group of red oil ointment, with 10 rats in each group. The wounds with an area of 3 cm×3 cm were modeled on the back of rats in each group. Except for the normal group, the low-dose group of red oil ointment, medium-dose group of red oil ointment and high-dose group of red oil ointment were applied with 4.744 mg, 94.875 mg and 189.750 mg red oil ointment gauze respectively. After continuous administration for 30 days, the mercury content in urine, blood, liver and kidney, and liver and kidney function indexes, such as serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine (CREA) were measured. Results: The mercury content in blood and urine in each dose group of red oil ointment were significantly higher than those in the normal group (P＜0.05, P＜0.01). The mercury content in liver and kidney in the medium-dose group of red oil ointment and high-dose group of red oil ointment were significantly higher than those in the normal group (P＜0.01). The mercury content in urine and kidney were dose-dependent (P＜0.01); compared with the normal group, the content of AST in the high-dose group of red oil ointment was significantly higher (P＜0.05), and the contents of BUN and CREA in the medium-dose group and high-dose group of red oil ointment were significantly higher (P＜0.05, P＜0.01). Conclusion: Long term and excessive administration of red oil ointment can cause mercury accumulation and liver and kidney toxicity in rats, but the daily average dose of 4.744 mg (equivalent to 230 mg of human clinical daily average dose) for 30 days' continuous administration has little effect on liver and kidney mercury accumulation and liver and kidney function, which can provide data reference for the clinical safety of red oil ointment.

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