| 赵锦燕 张毓宸 万芸 曾建伟 洪振丰.片仔癀肝宝对慢性肝损伤凋亡的影响[J].实用中西医结合临床,2017,17(1):1-2,50 |
| 片仔癀肝宝对慢性肝损伤凋亡的影响 |
| Effect of Pianzaihuang Ganbao on the Apoptosis of Chronic Liver Injury |
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| DOI： |
| 中文关键词: 肝损伤 凋亡 片仔癀肝宝 |
| 英文关键词: Liver injury Apotosis Pianzaihuang Ganbao |
| 基金项目:陈可冀中西医结合发展基金（编号：CKJ2013015） |
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| 中文摘要: |
| 目的：探讨片仔癀肝宝（肝宝）对CCl4诱导大鼠慢性肝损伤凋亡的影响。方法：60只SD大鼠随机分为正常对照组、模型组、西利宾胺组、肝宝低剂量组、肝宝中剂量组、肝宝高剂量组，每组10只。除正常对照组外，SD大鼠腹腔注射50% CCl4橄榄油溶液1 ml/kg，2次/周，连续8周。于造模第四周开始给药，设正常对照组（生理盐水）、模型组（生理盐水）、阳性药对照组（西利宾胺，50 mg/kg），肝宝低剂量组（150 mg/kg）、肝宝中剂量组（300 mg/kg）、肝宝高剂量组（600 mg/kg），1次/d，连续4周。于末次给药24 h后，HE染色观察肝宝对肝组织病理学的影响，TUNEL法观察肝组织凋亡情况，比色法检测Caspase-3和Caspase-9的变化，western blot检测肝宝对Bax和Bcl-2蛋白表达的影响。结果：HE染色发现，各用药组均可不同程度缓解CCl4诱导的肝损伤；肝宝抑制肝细胞凋亡；降低Caspase-3和Caspase-9的活力；下调Bax表达，上调Bcl-2表达。结论：片仔癀肝宝可以抑制CCl4诱导的肝细胞凋亡。 |
| 英文摘要: |
| Objective: To explore the effect of Pianzaihuang Ganbao (GB) on the apotosis of chronic liver injury induced by CCl4 in rats. Methods: Sixty Sprague–Dawley (SD) rats were randomly divided into control, model, silymarin, GB-low, GB-middle and GB-high groups, ten rats per group. Except the control group was received olive oil, the rest groups were intraperitoneally injected with CCl4 1 ml/kg, twice a week for 8 weeks constitutively. At the fourth week, the model and the control group were orally administration physiological saline (PS), while the silymarin (50 mg/kg), GB-Low (150 mg/kg), GB-middle (300 mg/kg), GB-high (600 mg/kg) were orally administrated in treatment group respectively for 4 weeks. Twenty-four hours after the last treatment, HE staining was used to observe the histopathological change in liver; the apoptosis was determined by TUNEL assay; Caspase-3 and Caspase-9 were detected; the protein expression of Bax and Bcl-2 were determined by western blot. Results: The results from HE staining found that all treatment could alleviate the injuried liver tissues differently; GB could inhibit the apotosis and decrease the activity of Caspase-3 and Caspase-9; furthermore, down-regulated the expression of Bax and up-regulated Bcl-2. Conclusion: GB could inhibit the apotosis induced by CCl4. |
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