赵锦燕 张毓宸 万芸 曾建伟 洪振丰.片仔癀肝宝对慢性肝损伤凋亡的影响[J].实用中西医结合临床,2017,17(1):1-2,50 |
片仔癀肝宝对慢性肝损伤凋亡的影响 |
Effect of Pianzaihuang Ganbao on the Apoptosis of Chronic Liver Injury |
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DOI: |
中文关键词: 肝损伤 凋亡 片仔癀肝宝 |
英文关键词: Liver injury Apotosis Pianzaihuang Ganbao |
基金项目:陈可冀中西医结合发展基金(编号:CKJ2013015) |
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中文摘要: |
目的:探讨片仔癀肝宝(肝宝)对CCl4诱导大鼠慢性肝损伤凋亡的影响。方法:60只SD大鼠随机分为正常对照组、模型组、西利宾胺组、肝宝低剂量组、肝宝中剂量组、肝宝高剂量组,每组10只。除正常对照组外,SD大鼠腹腔注射50% CCl4橄榄油溶液1 ml/kg,2次/周,连续8周。于造模第四周开始给药,设正常对照组(生理盐水)、模型组(生理盐水)、阳性药对照组(西利宾胺,50 mg/kg),肝宝低剂量组(150 mg/kg)、肝宝中剂量组(300 mg/kg)、肝宝高剂量组(600 mg/kg),1次/d,连续4周。于末次给药24 h后,HE染色观察肝宝对肝组织病理学的影响,TUNEL法观察肝组织凋亡情况,比色法检测Caspase-3和Caspase-9的变化,western blot检测肝宝对Bax和Bcl-2蛋白表达的影响。结果:HE染色发现,各用药组均可不同程度缓解CCl4诱导的肝损伤;肝宝抑制肝细胞凋亡;降低Caspase-3和Caspase-9的活力;下调Bax表达,上调Bcl-2表达。结论:片仔癀肝宝可以抑制CCl4诱导的肝细胞凋亡。 |
英文摘要: |
Objective: To explore the effect of Pianzaihuang Ganbao (GB) on the apotosis of chronic liver injury induced by CCl4 in rats. Methods: Sixty Sprague–Dawley (SD) rats were randomly divided into control, model, silymarin, GB-low, GB-middle and GB-high groups, ten rats per group. Except the control group was received olive oil, the rest groups were intraperitoneally injected with CCl4 1 ml/kg, twice a week for 8 weeks constitutively. At the fourth week, the model and the control group were orally administration physiological saline (PS), while the silymarin (50 mg/kg), GB-Low (150 mg/kg), GB-middle (300 mg/kg), GB-high (600 mg/kg) were orally administrated in treatment group respectively for 4 weeks. Twenty-four hours after the last treatment, HE staining was used to observe the histopathological change in liver; the apoptosis was determined by TUNEL assay; Caspase-3 and Caspase-9 were detected; the protein expression of Bax and Bcl-2 were determined by western blot. Results: The results from HE staining found that all treatment could alleviate the injuried liver tissues differently; GB could inhibit the apotosis and decrease the activity of Caspase-3 and Caspase-9; furthermore, down-regulated the expression of Bax and up-regulated Bcl-2. Conclusion: GB could inhibit the apotosis induced by CCl4. |
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