| 罗文艳 段和祥.蒌蒿乙酸乙酯部位体外抗乙型肝炎病毒的实验研究[J].实用中西医结合临床,2016,16(9):84-85 |
| 蒌蒿乙酸乙酯部位体外抗乙型肝炎病毒的实验研究 |
| Experimental study on Ethyl Acetate Fraction from Artemisia selengensis anti- HBV in vitro |
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| 中文关键词: 乙型肝炎病毒 蒌蒿乙酸乙酯部位 乙型肝炎病毒表面抗原 乙型肝炎病毒e抗原 |
| 英文关键词: HBV Ethyl Acetate Fraction from Artemisia selengensis HBsAg HBeAg |
| 基金项目:江西省卫生厅中医指导性计划（编号：2013B014）；江西省科技厅青年科学基金资助项目（编号：20122BAB215044） |
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| 中文摘要: |
| 目的：研究蒌蒿乙酸乙酯部位体外抗乙型肝炎病毒（HBV）的作用。方法：蒌蒿药材用95%乙醇提取后，用乙酸乙酯萃取获得有效部位，作用于HBV-DNA转染的HepG2.2.15细胞上。采用MTT法检测乙酸乙酯部位对HepG2.2.15细胞的半数抑制浓度（IC50），用ELISA法观察乙酸乙酯部位对HepG2.2.15细胞分泌HBsAg和HBeAg表达的抑制作用。结果：蒌蒿乙酸乙酯部位对HepG2.2.15细胞有一定的细胞抑制作用，对HepG2.2.15细胞分泌HBsAg和HBeAg表达有不同程度的抗原抑制作用。结论：蒌蒿乙酸乙酯部位有较强的抗乙型肝炎病毒作用。 |
| 英文摘要: |
| Objective: To study the effect of ethyl acetate from Artemisia selengensis anti-HBV in vitro. Methods: After the Artemisia selengensis were extracted with 95% ethanol, extracted with ethyl acetate. Then the effective part was acted on HBV-DNA transfected HepG2.2.15 cells. MTT assay was used to detect ethyl acetate fraction inhibitory concentration 50% of HepG2.2.15 cells. ELISA method was used to investigate the suppressor effect on HBsAg and HBeAg in the cultured HepG2.2.15 cells which were gene transferred with HBV. Results: Ethyl acetate fration from Artemisia selengensis have a certain cell inhibitory effect on HepG2.2.15 cells. HepG2.2.15 cells the secretion of HBsAg and HBeAg express different degrees of antigen inhibition. Conclusion: The ethyl acetate from Artemisia has strong anti HBV effect. |
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