| 周美英 魏林珍 王海琳 张爱笠 甄洁玉.苦参碱诱导宫颈癌SiHa细胞凋亡的相关机制研究[J].实用中西医结合临床,2016,16(8):1-4 |
| 苦参碱诱导宫颈癌SiHa细胞凋亡的相关机制研究 |
| Effects of Matrine on Apoptosis of Human Cervical Cancer Cell SiHa and Its Mechanism |
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| DOI： |
| 中文关键词: 苦参碱 宫颈癌 细胞增殖 细胞凋亡 Z-DEVD-FMK Caspase-3 |
| 英文关键词: Matrine Cervical cancer Proliferation Apoptosis Z-DEVD-FMK, Caspase-3 |
| 基金项目:国家自然科学基金（编号：H1621）；甘肃省自然基金（编号：1208RJZA109） |
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| 中文摘要: |
| 目的：观察苦参碱体外诱导宫颈癌SiHa细胞凋亡效应并对其可能的作用机制进行初步探讨。方法：将不同浓度（0.00、0.25、0.50、1.00、2.00 mg/ml）的苦参碱分别作用于体外培养的宫颈癌细胞，在不同时间点（24、48、72 h），采用MTT法检测细胞的增殖情况；采用Annexin-Ⅴ和PI双染法在流式细胞仪上检测细胞的凋亡率；采用Western blot检测细胞中凋亡相关蛋白Caspase-3的表达。结果：各组苦参碱对SiHa细胞的增殖均有抑制作用，MTT和流式细胞结果显示，不同浓度的苦参碱对细胞生长抑制率与对照组相比存在明显差异（P＜0.05），呈剂量效应正相关及时间效应正相关。加入Caspase-3抑制剂Z-DEVD-FMK后，各组细胞凋亡率与只加苦参碱组相比凋亡率下降。Western blot检测结果显示Caspase-3蛋白水平随苦参碱浓度的提高而增加（P＜0.05）。结论：苦参碱可诱导宫颈癌SiHa细胞的增殖，促进其凋亡，其作用机制可能与提高Caspase-3活性有关。 |
| 英文摘要: |
| Objective: To observe the role of matrine (Mat) induced apoptosis of human cervical cancer SiHa cells and its mechanism. Methods: SiHa cells were exposed to the concentrations of matrine 0.00, 0.25, 0.50, 1.00 and 2.00 mg/ml for 24, 48, 72 h. Cell proliferation was detected by MTT assay. Apoptosis was determined by double stained assay with Annexin-Ⅴand PI. Caspase-3 protein level was determined with western blot assay. Results: After SiHa cells were cocultured with matrine in vitro, the cell proliferation was inhibited in time and dose dependent manner. When the SiHa cells were cultured for 24, 48, 72 h by matrine 0.50, 1.00, 2.00 mg/ml, the total apoptosis rate was increased, which had ststistical significance compared with control group(P＜0.05). The addition of the caspase-3 inhibitor Z-DEVD-FMK, the apoptotic rate and increase of matrine group decreased apoptosis rate. And the caspase-3 protein level increased with the increase of the concentration of matrine (P＜0.05). Conclusion: Matrine can inhibit the proliferation and induce the apoptosis of SiHa cells in vitro, one of its possible mechanisms is that matrine increasing caspase-3 activity. |
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