| 王海东 谢建祥 林伟青.Eupatilin对黄嘌呤氧化酶活性抑制的实验研究[J].实用中西医结合临床,2015,15(9):87-89 |
| Eupatilin对黄嘌呤氧化酶活性抑制的实验研究 |
| Study on Inhibitory Effect of Eupatilin on Xanthine Oxidase Activity |
| |
| DOI： |
| 中文关键词: 异泽兰黄素 黄嘌呤氧化酶 黄嘌呤 活性抑制 |
| 英文关键词: Eupatilin Xanthine oxidase (XO) Xanthine Inhibitory effect |
| 基金项目: |
|
| 摘要点击次数: 945 |
| 全文下载次数: 702 |
| 中文摘要: |
| 目的：通过体外酶学试验分析Eupatilin对黄嘌呤氧化酶活性的影响及抑制机理。方法：利用紫外分光光度计观测尿酸生成导致的吸光度变化值，测定波长为292 nm，并以黄嘌呤为底物根据测定结果绘制Lineweaver-Burk和Dixon图。结果：Eupatilin对黄嘌呤氧化酶表现很强的抑制作用，其Ki值为0.128 6 ?滋g/ml，抑制类型为混合型；而别嘌呤醇作为对照，其Ki值为0.62 ?滋g/ml，抑制类型为竞争性抑制。结论：Eupatilin具有明显的黄嘌呤氧化酶抑制活性。 |
| 英文摘要: |
| Objective: To research the influence and inhibitory mechanism of Eupatilin on the activity of Xanthine Oxidase by vitro enzymatic test. Methods: Determined the absorbance changes caused by the formation of uric acid value by using UV spectrophotometer, the detection wavelength was 292 nm, according to the measured results, draw the Lineweaver Burk and Dixon plots with Xanthine as substrate. Results: Eupatilin had strong inhibition on Xanthine Oxidase, the Ki value was 0.128 6 ?滋g/ml, the type of inhibition is of mixed type; and with Allopurinol as a reference, the Ki value was 0.62 ?滋g/ml, the type of inhibition was competitive inhibition. Conclusion: Eupatilin has the obvious inhibitory activity of Xanthine Oxidase. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
