| 熊声贺 王琴琴 黄新城.应用EP7-A2方案评价循环酶法同型半胱氨酸试剂对其他生化检测项目的干扰[J].实用中西医结合临床,2011,(4):67-68 |
| 应用EP7-A2方案评价循环酶法同型半胱氨酸试剂对其他生化检测项目的干扰 |
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| DOI： |
| 中文关键词: 同型半胱氨酸 糖化血清蛋白 生化分析仪 携带交叉污染 |
| 英文关键词: Homocysteine(HCY) Saccharification serum protein(GSP) Biochemical analyzer Carry cross contamination |
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| 中文摘要: |
| 目的：研究循环酶法同型半胱氨酸检测试剂对尿酸等项目检测的干扰。方法：用CLSI EP7-A2方案，评估循环酶法同型半胱氨酸试剂在生化分析仪中，因携带交叉污染对常用项目检测的干扰。结果：检测结果显示，在模拟污染率0.1%~0.5%范围内， ALT、AST从0.1%污染率开始，结果均超出（X-1.96s）范围；GSP从0.2%污染率开始，结果均超出（X+1.96s）范围；UA从0.3%污染率开始，结果均超过（X-1.96s）范围；Mg从0.3%污染率开始，结果均超出（X+1.96s）范围；其他15项目检测结果均在（X±1.96s）范围内。结论：生化分析仪携带污染率在0.5%的允许范围内，循环酶法同型半胱氨酸检测试剂可能对同一检测系统中，糖化血清蛋白（GSP）等5项检测造成干扰，而对总胆红素等其他15项检测不造成干扰。 |
| 英文摘要: |
| Objective:To study uric acid et.detecting interferenced of the circulation enzymatic homocysteine reagent.Methods:Using CLSI EP7-A2 scheme, evaluate circular enzymatic homocysteine reagent in biochemistry analyzer,because carry cross contamination of interference to commonly used analysis items detection.Results:The simulation tests showed that the rate of pollution within 0.1%~0.5%,from 0.1% rate of pollution,the ALT and AST test results exceed (X-1.96s) limit; from 0.2% rate of pollution,the GSP test results exceed (X+1.96s) limit;from 0.3% rate of pollution,the uric acid test results exceed(X-1.96s) limit;from 0.3% rate of pollution,the Mg test results exceed(X+1.96s) limit.Other 15 projects are testing results within (X±1.96s).Conclusion:Biochemical analyzer to carry the rate of pollution in the range of allowable 0.5%,circulation enzymatic homocysteine reagent in the same detection system, saccharification serum protein (GSP) and five test cause interference, but to total bilirubin and other 15 test don't cause interference. |
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