耿国军 姜杰 杜好信 钱文轩 张义 杨丽华 陈端扬 陈隽鹏.黄芪对肺腺癌细胞生长抑制的实验研究[J].实用中西医结合临床,2011,(1):4-5 |
黄芪对肺腺癌细胞生长抑制的实验研究 |
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DOI: |
中文关键词: 黄芪 肺腺癌 SPC-A-1 MTT法 生长抑制 |
英文关键词: Astragalus Lung adenocarcinoma cel1 SPC-A-1 Thiazolyl blue Growth suppression |
基金项目:福建省自然科学基金项目:闽科计[2008]59号文 |
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中文摘要: |
目的:研究分化诱导剂黄芪(Astragalus)在体外对人肺腺癌SPC-A-1细胞生长抑制的影响。方法:用不同浓度的黄芪分别处理肺腺癌SPC-A-1细胞后,镜下观察癌细胞的生长情况;测定软琼脂克隆形成率;MTT(噻唑蓝)法测定生长抑制率。结果:不同浓度黄芪处理后细胞的生长明显受到抑制,细胞生长抑制率随黄芪的浓度增加而增加。结论:黄芪对肺腺癌SPC-A-1细胞的生长有明显的抑制作用。 |
英文摘要: |
Objective:To study the activity of the growth suppression effects with Astragalus on SPC-A-1 human lung adenocarcinoma cel1.Methods:Different concentrations of Astragalus were used in the culture of SPC-A-1 cells. Determination of clone forming ability on soft agar and growth suppression effects with Astragalus were measured by a tetrazolium-based volorimetric assay (MTT assay).Results:Proliferation of SPC-A-1 cells was inhibited effectively after being treated by different concentrations of Astragalus,the rate of growth suppression was increasing with increased concentration of Astragalus.Conclusion:Astragalus have the significant effect on inhibiting on SPC-A-1 human lung adenocarcinoma cel1. |
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