| 陈松劲 王兆丰 吴晓燕.HBV-DNA定量与HBV-M检测结果的对比研究[J].实用中西医结合临床,2007,(4):60-61 |
| HBV-DNA定量与HBV-M检测结果的对比研究 |
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| DOI： |
| 中文关键词: 荧光定量PCR HBV-DNA HBV-M ELISA |
| 英文关键词: Fluorescent quantitative PCR HBV-DNA HBV-M ELISA |
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| 中文摘要: |
| 目的：了解HBV-DNA定量检测与HBV血清标志物定性或定量检测结果之间的关系。方法：运用荧光定量PCR（FQ-PCR）检测患者血清中的HBV-DNA，同时运用ELISA法或时间分辨荧光免疫法检测HBV血清标志物，并对结果进行对比研究。 结果：HBsAg(+)HBeAg(+)HBcAb(+)组患者血清HBV-DNA检出率最高为94.62%（176/186），HBsAg(+)HBcAb(+)组患者血清HBV-DNA检出率为42.86%（30/70），HBsAg(+)HBeAb(+)HBcAb(+)组患者血清HBV-DNA检出率为26.17%（56/214），HBsAg（+）HBeAg(+)HBcAb(+)组显著高于其它各组（P <0.01）。结论：血清HBV-DNA水平与HBV血清标志物的表现模式有关，HBeAg与HBV-DNA 有高度的相关性。HBV-DNA与HBV-M结合能全面了解HBV感染、复制及传染性，也为指导治疗提供客观依据。 |
| 英文摘要: |
| Objective:To explore the relationship between detection of HBV-DNA and HBV serum markers. Methods:HBV-DNA was detected with fluorescent quantitative PCR(FQ-PCR) and HBV was routinely determined with ELISA or time-resolved immunofluorometric assay. Results:The detecting rate of HBV-DNA was 94.62%(176/186) in patients of HBsAg(+)HBeAg(+)HBcAb(+),42.86%(30/70) in patients of HBsAg(+)HBcAb(+),26.17%(56/214) in patients of HBsAg(+)HBeAb(+)HBcAb(+). The parameter was significantly higher in HBsAg(+)HBeAg(+)HBcAb(+) than in others(P <0.01).Conclusion:There is a correlation between HBV-DNA and HBV-M, especialy between HbeAg and HBV-DNA .Both HBV-DNA and HBV-M determination could reflect the infection、replication of HBV and provide objective evidences for diagnosis and treatment monitoring of hepatitis B. |
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