黄芩素通过线粒体途径影响胃癌耐药细胞AGS/DDP的增殖和凋亡

喻春梅; 谢丹丹

文章摘要

黄芩素通过线粒体途径影响胃癌耐药细胞AGS/DDP的增殖和凋亡

Baicalein affects the proliferation and apoptosis of gastric cancer drug-resistant AGS/DDP cells through mitochondrial pathway

投稿时间：2025-02-24 修订日期：2025-03-10

DOI：

中文关键词: 黄芩素线粒体胃癌顺铂耐药

英文关键词: Baicalein Mitochondria Gastric cancer Cisplatin resistance

基金项目:江西省中医药管理局科技计划项目(编号：2023A0040)；江西省教育厅科学技术研究项目(编号：GJJ2208204)

作者	单位	邮编
喻春梅	南昌医学院江西南昌	330052
谢丹丹^*	江西省儿童医院	330038

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中文摘要:

目的：探讨黄芩素对胃癌耐药细胞AGS/DDP增殖和凋亡的影响及机制。方法：不同浓度的黄芩素处理AGS/DDP细胞48h；采用CCK8法检测细胞增殖抑制率；流式细胞术测定细胞凋亡率；测定细胞内的ATP含量及细胞线粒体膜电位（MMP）；电镜下观察培养的胃癌细胞线粒体的形态变化；RT-qPCR方法检测胃癌细胞OPA1和OMA1的mRNA表达水平。结果：与对照组相比，黄芩素显著抑制AGS/DDP细胞增殖和促进其凋亡，以及ATP水平和线粒体膜电位降低（P<0.05）。与胃癌细胞AGS相比，AGS/DDP细胞线粒体呈长梭形及线粒体融合趋势明显，同时OPA1mRNA表达显著增高，而OMA1mRNA表达显著降低（P<0.001）。结论：黄芩素可能通过线粒体途径抑制胃癌耐药细胞的增殖和促进其凋亡。

英文摘要:

Objective: To investigate the effects and mechanisms of baicalein on the proliferation and apoptosis of drug-resistant gastric cancer AGS/DDP cells. Methods: AGS/DDP cells were treated with different concentrations of baicalein for 48 hours. The inhibi-tion rate of cell proliferation was detected by CCK8 assay. The apoptosis rate was de-termined by flow cytometry. The intracellular ATP content and mitochondrial mem-brane potential (MMP) were measured. The morphological changes of mitochondria in cultured gastric cancer cells were observed under electron microscopy. The mRNA expression levels of OPA1 and OMA1 in gastric cancer cells were detected by RT-qPCR. Results: Compared with the control group, baicalein significantly inhibited the proliferation and promoted the apoptosis of AGS/DDP cells, as well as decreased the ATP level and mitochondrial membrane potential (P < 0.05). Compared with AGS cells, AGS/DDP cells showed a long spindle shape and a significant trend of mito-chondrial fusion, while the mRNA expression of OPA1 was significantly increased and that of OMA1 was significantly decreased (P < 0.001). Conclusion: Baicalein can inhibit the proliferation and promote the apoptosis of drug-resistant gastric cancer possibly cells through the mitochondrial pathway.

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