| 史亚波,周梦娜,刘华兵,殷俊翔,曹振军,龚帅昌.康莱特注射液对人胆管癌细胞 RBE 和 HCCC9810生长、凋亡、迁移的影响[J].实用中西医结合临床,2023,23(11):1-5,13 |
| 康莱特注射液对人胆管癌细胞 RBE 和 HCCC9810生长、凋亡、迁移的影响 |
| The Effects of Kanglaite Injection on the Growth, Apoptosis and Migration of Human Cholangiocarcinoma Cancer Cells RBE and HCCC9810 |
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| DOI： |
| 中文关键词: 人胆管癌细胞 康莱特注射液 生长 凋亡 迁移 |
| 英文关键词: Human cholangiocarcinoma cell Kanglaite injection Growth Apoptosis Migration |
| 基金项目:江西省中医药管理局资助项目(编号:2020B0362) |
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| 中文摘要: |
| 目的:探讨康莱特注射液对人胆管癌细胞 RBE 和 HCCC9810 生长、凋亡、迁移的影响。 方法:使用康莱特
注 射 液 处 理 人 胆 管 癌 细 胞 RBE 和 HCCC9810 , 运 用 CCK-8 法 检 测 康 莱 特 注 射 液 对 人 胆 管 癌 细 胞 RBE 和
HCCC9810 的增殖情况,流式细胞仪检测两者的凋亡率, Transwell 实验检测两者的迁移能力,通过 Western%Blot 检测
两者信号通路中关键蛋白 Caspase-3 、 Bcl-2 和 Bax 的表达情况。 结果:较之空白对照组,随着康莱特药物作用时间的
延 长 ,两 者 细 胞 的 生 长 增 殖 程 度 逐 渐 受 到 抑 制 ( P <0.05 ),在 作 用 96%h 时 达 到 较 好 抑 制 状 态 ; Transwell 实 验 结 果 显
示 , 人 胆 管 癌 细 胞 RBE 和 HCCC9810 经 康 莱 特 处 理 后 迁 移 数 量 减 少 ( P <0.05 ); 实 验 组 人 胆 管 癌 细 胞 RBE 和
HCCC9810 凋 亡 率 明 显 增 加 ( P<0.05 ), 两 者 信 号 通 路 中 关 键 蛋 白 Caspase-3 、 Bax 蛋 白 表 达 明 显 上 调 ( P<0.05 ),
Bcl-2 蛋白表达下调( P<0.05 )。 结论:康莱特注射液可以抑制人胆管癌细胞 RBE 和 HCCC9810 的生长和迁移能力,
促进其凋亡,机制可能与其能上调凋亡信号通路关键蛋白 Caspase-3 、 Bax 和下调 Bcl-2 蛋白表达有关。 |
| 英文摘要: |
| Objective: To investigate the effects of Kanglaite injection on the growth, apoptosis and migration of human cholangiocarcinoma cells RBE and HCCC9810. Methods: Kanglaite injection was used to treat human cholangiocarcinoma cells RBE and HCCC9810, the proliferation of Kanglaite injection on human cholangiocarcinoma cells RBE and HCCC9810 were detected by CCK-8 method, the apoptosis rate of both were detected by flow cytometry, and the migration ability of both were detected by Transwell assay. The expressions of key proteins Caspase-3, Bcl-2 and Bax in both signaling pathways were detected by Western blot. Results: Compared to the control group, the growth and proliferation degree of both cells were gradually inhibited while the prolongation of Kanglaite action time(P＜0.05), and reached a better inhibitory state after 96 hour of treatment. Transwell assay showed that the number of migration of human bile duct cancer cells RBE and HCCC9810 decreased after Kanglaite treatment(P＜0.05). The apoptosis rate of RBE and HCCC9810 of bile duct cancer cells in the experimental group was significantly increased(P＜0.05), the expressions of key proteins Caspase-3 and Bax in both signaling pathways were significantly up-regulated(P＜0.05), and the expression of Bcl-2 protein was down-regulated(P＜0.05). Conclusion: Kanglaite injection can inhibit the growth and migration of RBE and HCCC9810 of human cholangiocarcinoma cells, and promote their apoptosis. The mechanism may be related to the up-regulation of apoptosis signaling pathway key proteins Caspase-3 and Bax and down-regulation of Bcl-2 protein expression. |
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