| 张婷，曾建伟，陈俊，戴雨婷，郑若曦，吴广文.洗腿又方治疗骨关节炎的网络药理学研究[J].实用中西医结合临床,2022,22(7):7-12,38 |
| 洗腿又方治疗骨关节炎的网络药理学研究 |
| Network Pharmacological Research of Xituiyou Prescription in the Treatment of Osteoarthritis* |
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| DOI： |
| 中文关键词: 骨关节炎 洗腿又方 网络药理学 分子对接 |
| 英文关键词: Osteoarthritis Xituiyou prescription Network pharmacology Molecular docking |
| 基金项目:陈可冀中西医结合发展基金（编号：CKJ2021014） |
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| 中文摘要: |
| 目的：通过生物信息学、网络药理学和分子对接技术探讨洗腿又方治疗骨关节炎的药理学机制。方法：从基因表达综合数据库（GEO）中获取骨关节炎滑膜的差异表达基因，使用TCMSP、HERB、TCMID等数据库搜集洗腿又方的活性成分及其作用靶点，并与疾病靶点取交集。通过STRING数据库及Cytoscape 3.8.2软件对交集靶点进行蛋白互作和富集分析，最终将关键活性成分与关键靶点进行分子对接。结果：获得骨关节炎滑膜的差异表达基因 2 072个，洗腿又方的活性成分20种，其治疗骨关节炎的靶点30个，进一步通过蛋白互作筛选获得HIF1A、CASP8、RAF1等关键靶点，富集分析发现洗腿又方主要通过干预细胞周期、炎症和内分泌等信号通路治疗骨关节炎，分子对接结果显示，关键有效成分芹黄素、槲皮素分别与关键靶点HIF1A、CASP8有良好的对接活性。结论：对洗腿又方治疗骨关节炎的可能活性成分、靶点及作用通路有了初步认识，为其应用于骨关节炎的治疗提供了理论依据。 |
| 英文摘要: |
| Objective: To explore the pharmacological mechanism of Xituiyou prescription in the treatment of osteoarthritis through bioinformatics, network pharmacology and molecular docking technology. Methods: The differentially expressed genes of osteoarthritis synovium were obtained from the Gene Expression Comprehensive Database (GEO), and the active components and their action targets of Xituiyou prescription were collected by using TCMSP, HERB, TCMID databases, and intersected with the disease targets. Through the STRING database and Cytoscape 3.8.2 software, the protein interaction and enrichment analysis of the intersection targets were carried out, and finally the key active components were moleculary docked with the key targets. Results: 2 072 differentially expressed genes in the osteoarthritis synovium, 20 active components of Xituiyou prescription, and 30 targets for the treatment of osteoarthritis were obtained. Key targets such as HIF1A, CASP8 and RAF1 were further obtained through protein interaction screening. Enrichment analysis found that Xituiyou prescription mainly treated osteoarthritis by interfering with signaling pathways such as cell cycle, inflammation and endocrine. The molecular docking results showed that the key active components were apigenin quercetin had good docking activities with the key targets HIF1A and CASP8, respectively. Conclusion: We have a preliminary understanding of the possible active components, targets and action pathways of Xituiyou prescription in the treatment of osteoarthritis, which provides a theoretical basis for its application in the treatment of osteoarthritis. |
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