| 吴辉渊.基于网络药理学探讨肝积汤治疗肝癌的分子机制[J].实用中西医结合临床,2022,22(9): |
| 基于网络药理学探讨肝积汤治疗肝癌的分子机制 |
| Discussion on the Molecular Mechanism of Ganji-Soup in the Treatment of Liver Cancer Based on Network Pharmacology |
| 投稿时间：2022-05-17 修订日期：2022-05-27 |
| DOI： |
| 中文关键词: 肝积汤 肝癌 网络药理学 |
| 英文关键词: Ganji-Soup Liver cancer Network Pharmacology |
| 基金项目:江西省中医药管理局科技计划（2021A149） |
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| 中文摘要: |
| 目的 探讨基于网络药理学的肝积汤活性成分、治疗靶点及分子机制。方法肝积汤的活性成分及作用靶点从TCMSP数据库筛选,肝癌相关靶点从GeneCard数据库获取,运用String数据库对共同靶点做蛋白互作网络分析,GO和KEGG富集分析采用Bioconductor生物信息软件包进行,“成分－靶点－通路”网络图采用Cytoscape制作。结果 从 TCMSP 中获得肝积汤有效活性成分 80种以及249 个药效分子靶点。与检索到的885 个肝癌相关靶点取交集,得到95个共同靶点。蛋白互作网络分析显示有TP53、VEGFA、AKT1等15个核心靶点。GO 功能分析表明,这些靶点主要富集在细胞对化学应激的反应,细胞对氧化应激的反应,对脂多糖反应等；涉及细胞周期蛋白依赖性蛋白激酶全酶复合物、丝氨酸/苏氨酸蛋白激酶复合物,蛋白络氨酸激酶等,主要涉及DNA结合转录因子结合、RNA聚合酶Ⅱ特异性DNA结合转录因子结合、泛素样蛋白连接酶结合。KEGG 富集分析结果显示,肝积汤作用靶点富集在相关肝癌发生与发展的通路上,如PI3K-Akt信号通路、乙型肝炎病毒信号通路、人巨细胞病毒感染信号通路。结论 槲皮素、木犀草素、山柰酚等可能为肝积汤治疗肝癌的关键成分,TP53、VEGFA、AKT1等为核心靶点,PI3K-Akt、乙型肝炎病毒、人巨细胞病毒感染等通路为主要作用的通路。 |
| 英文摘要: |
| Objective To investigate the active ingredients, therapeutic targets and molecular mechanisms of Ganji-Soup based on network pharmacology. MethodsThe active ingredients and targets of Ganji-Soup were screened from TCMSP database, liver cancer-related targets were obtained from GeneCard database, protein interaction network analysis of common targets was performed by String database, GO and KEGG enrichment analysis was carried out by Bioconductor bioinformatics software package, and the "component-target-pathway" network diagram was prepared by Cytoscape. Results 80 active ingredients and 249 pharmacodynamic molecular targets of Ganji-Soup were obtained from TCMSP. It intersected with 885 liver cancer-related targets retrieved to obtain 95 common targets. Protein interaction network analysis showed 15 core targets such as TP53, VEGFA, AKT1, etc. GO functional analysis showed that these targets were mainly enriched in cell responses to chemical stress, cell responses to oxidative stress, responses to lipopolysaccharides, etc. It involves cyclin-dependent protein kinase whole enzyme complex, serine/threonine protein kinase complex, proteronine kinase, etc., mainly involving DNA-binding transcription factor binding, RNA polymerase II. specific DNA binding transcription factor binding, and ubiquitin-like protenesin binding. The results of KEGG enrichment analysis showed that hepatic soup was enriched in pathways related to the occurrence and development of liver cancer, such as PI3K-Akt signaling pathway, hepatitis B virus signaling pathway, and human cytomegalovirus infection signaling pathway. Conclusion Quercetin, luteolin, kaempferol, etc. may be the key components of Ganji-Soup in the treatment of liver cancer, TP53, VEGFA, AKT1, etc. are the core targets, and channels such as PI3K-Akt, hepatitis B virus, and human cytomegalovirus infection are the main pathways. |
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