| 王利勤,熊鸣峰,王腾宇,万海同,熊墨年,吴春红.基于网络药理学探讨附子配伍甘草治疗慢性心力衰竭的作用机制[J].实用中西医结合临床,2022,22(7): |
| 基于网络药理学探讨附子配伍甘草治疗慢性心力衰竭的作用机制 |
| Study Mechanisms ofAconite and Licorice on Chronic Heart Failure Based on Network Pharmacology |
| 投稿时间：2022-03-25 修订日期：2022-05-10 |
| DOI： |
| 中文关键词: 附子甘草配伍 慢性心力衰竭 蛋白质相互作用 生物信息学 网络药理学 |
| 英文关键词: compatibility of Fuzi and Gancao, Chronic Heart Failure, Protein Interaction, Bioinformatics, Network Pharmacology |
| 基金项目:(编号：No.81904087)；2.2019 年全国中医药创新骨干人才培训项目(国中医药办人教函[2019]128 号)；3.2020 年江西省中医药中青年骨干人才培养项目(第一批)(赣中医科教字[2020]2 号)；4.江西省科技厅重点研发项目(编号：No.20203BBE52W010)；5.熊墨年国医名师传承工作室[赣中医药综合字(2021)12号]。 |
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| 中文摘要: |
| 目的：基于网络药理学阐明附子甘草配伍对慢性心力衰竭的作用机制。方法：通过数据库 TCMSP、TCM、STITCH 以及 OMIM、 GeneCard 从附子甘草配伍的化学成分、成分靶点以及疾病靶点分别进行检索。再对相关疾病靶点和药物潜在靶点进行分析,制作韦恩图,筛选出共同交集。分别在 David 数据库和 Bioconductor 数据库中对合并所得的候选靶点进行 GO 富集分析和 KEGG 通路注释分析。结果：本研究共筛选出附子甘草配伍中 81 个化合物,对应的成分靶点 169 个。同时通过对慢性心力衰竭的检索得到 8668 个对应靶点,通过制作韦恩图了解到附子甘草配伍药对的潜在靶点与慢性心力衰竭的潜在靶点共有 154 个交集,5 条相关的信号通路。结论：附子甘草配伍治疗慢性心力衰竭的作用机制可能与调节 AKT、 MAPK 等相关信号通路有关,其中 AKT 是其主要信号通路。且附子甘草配伍的治疗慢性心力衰竭作用与细胞凋亡、IL-6、TNF 等细胞因子的表达密切相关,表示细胞凋亡、IL-6、TNF 可能是附子甘草配伍治疗慢性心力衰竭的重要作用靶点。 |
| 英文摘要: |
| Objective: To elucidate the anti chronic heart failure mechanisms of compatibility of Fuzi and Gancao. Methods: First, we used TCMSP, TCM, STITCH, OMIM and GeneCard databases to search the chemical components, component targets and disease targets of compatibility of Fuzi and Gancao. Then, we analyzed the targets of all the diseases and potential targets of drugs, and made the Wayne map, and screened out the common intersection. Lastly, the Go enrichment analysis and KEGG path annotation analysis were performed on the merged candidate targets in the David database and Bioconductor database respectively. Results: In this study, 81 compounds of compatibility of Fuzi and Gancao were screened out, and 169 corresponding component targets were found. At the same time, 8668 corresponding targets were obtained through the retrieval of chronic heart failure. By making Wayne map, we found that there were 154 intersections and 5 related signal pathways between compatibility of Fuzi and Gancao potential targets and chronic heart failure potential targets. Conclusion: The mechanism of compatibility of Fuzi and Gancao on chronic heart failure may be related to the regulation of AKT, MAPK and other associated signaling pathways, and the AKT pathway may be the main signaling pathway. Meanime, the effects on chronic heart failure of compatibility of Fuzi and Gancao is closely correlated with the expression of apoptosis, IL-6, TNF and other cytokines, which indicated that apoptosis, IL-6, TNF may be important targets on the treatment of compatibility of Fuzi and Gancao on chronic heart failure. |
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