基于整合药理学平台预测黄芩-黄连对溃疡性结肠炎的作用靶点

肖戈; 陆文洪

文章摘要

肖戈，陆文洪.基于整合药理学平台预测黄芩-黄连对溃疡性结肠炎的作用靶点[J].实用中西医结合临床,2022,22(2):1-6

基于整合药理学平台预测黄芩-黄连对溃疡性结肠炎的作用靶点

Prediction of Target of Huangqin-Huanglian Drug Pair in the Treatment of Ulcerative Colitis Based on Integrated Pharmacology Platform

DOI：

中文关键词: 溃疡性结肠炎黄芩-黄连药对网络药理学作用机制

英文关键词: Ulcerative colitis Huangqin-Huanglian drug pair Network pharmacology Mechanism of action

基金项目:湖南省教育厅科学研究项目（编号：20C1422）

作者	单位
肖戈，陆文洪	湖南中医药大学第二附属医院

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中文摘要:

目的：基于网络药理学，预测黄芩-黄连药对治疗溃疡性结肠炎（Ulcerative Colitis, UC）的有效活性成分及其潜在的作用机制。方法：以“黄芩-黄连”药对为检索词，在中药系统药理学数据库与分析平台（TCMSP）中按条件查找，逐步筛选出相应活性成分及潜在作用靶点；然后在GeneCards数据库中查找出UC的疾病靶基因；通过软件分析，得出活性成分与靶点之间，以及疾病各靶点蛋白之间的相互关系，并通过GO和KEGG富集分析获得黄芩-黄连药对治疗UC的潜在作用靶点和通路。结果：通过查找有效成分，分析出黄芩-黄连药对中有效活性成分共51个，其中与UC密切相关的靶点共136个；通过GO富集分析得到2 226条与生物过程相关的条目，采用KEGG通路富集分析得到165条潜在信号通路。结论：磷脂酰肌醇3-激酶（PI3K）和AKT蛋白酶 b （PI3K-AKT），丝裂原活化蛋白激酶（MAPK）可能是黄芩-黄连药对治疗UC调控的主要通信号通路，涉及的靶点包括肿瘤蛋白53（TP53）、丝/苏氨酸蛋白激酶（AKT1）、转录因子JUN、丝裂原活化蛋白激酶（MAPK1）、肿瘤坏死因子（TNF）等，为实验验证该药对治疗UC的作用机制提供了证据。

英文摘要:

Objective: To predict the effective active components and potential mechanism of Huangqin-Huanglian drug pair in the treatment of ulcerative colitis (UC) based on network pharmacology. Methods: Took "Huangqin-Huanglian" drug pair as the key word, the corresponding active components and potential targets were gradually screened out according to the conditions in the pharmacology database and analysis platform of Traditional Chinese medicine System (TCMSP). Then, the disease target genes of UC were found in GeneCards database. Through software analysis, the relationship between active components and targets, as well as the target proteins of diseases was obtained, and the potential target and pathway of Huangqin-Huanglian drug pair on the treatment of UC were obtained through GO and KEGG enrichment analysis. Results: By searching for the effective components, 51 effective active components of Huangqin-Huanglian drug pair were analyzed, including 136 targets closely related to UC; 2 266 entries related to biological processes were obtained by GO enrichment analysis, and 165 potential signal pathways were obtained by KEGG pathway enrichment analysis. Conclusion: PI3K-AKT and MAPK may be the main signaling pathways regulated by Huangqin-Huanglian drug pair in the treatment of UC. The targets involved include TP53, AKT1, JUN, MAPK1 and TNF, which provides evidence for the experimental verification of the mechanism of the drug in the treatment of UC.

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