| 周克平，宋文龙，肖林秀，吴芳芳.四君子汤通过影响TNF-α、IL-6干预脾虚大鼠胃黏膜损伤的实验研究[J].实用中西医结合临床,2021,21(19):4-7,16 |
| 四君子汤通过影响TNF-α、IL-6干预脾虚大鼠胃黏膜损伤的实验研究 |
| Experimental Study of Sijunzi Decoction Intervening Gastric Mucosal Injury in Spleen Deficiency Rats by Influencing TNF-α and IL-6 |
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| DOI： |
| 中文关键词: 四君子汤 脾气虚证 胃黏膜 疾病模型 |
| 英文关键词: Sijunzi decoction Spleen deficiency Gastric mucosal Disease model |
| 基金项目:浙江省杭州市建德市科技发展计划基金项目（编号：2019NY14） |
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| 中文摘要: |
| 目的：研究四君子汤对脾虚大鼠胃黏膜肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）表达及损伤指数的影响，探讨其对胃黏膜保护相关作用机制。方法：将大鼠随机分成空白对照组、脾虚模型组、四君子汤组、西药组、自然康复组，以小承气汤灌胃联合饮食失节进行脾虚大鼠造模，经药物灌胃干预4周后取出胃组织，运用Guth法计算各组胃黏膜损伤指数，ELISA法检测TNF-α、IL-6表达水平，分析胃黏膜损伤情况。结果：与空白对照组相比，造模后大鼠TNF-α、IL-6水平升高，胃黏膜损伤明显；与脾虚模型组、自然康复组比较，四君子汤组及西药组IL-6、TNF-α均降低，且胃黏膜损伤明显减轻，差异显著有统计学意义（P＜0.01），但两药物治疗组间比较无显著差异（P＞0.05）。结论：四君子汤能显著改善脾虚证大鼠胃黏膜组织的溃疡损伤，可能通过下调TNF-α、IL-6的表达来修复胃黏膜从而改善脾虚。 |
| 英文摘要: |
| Objective: To investigate the effects of Sijunzi decoction on the expression of tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) and injury index in gastric mucosa of rats with spleen deficiency, and to explore its protective mechanism of the gastric mucosa. Methods: The rats were randomly divided into blank control group, spleen deficiency model group, Sijunzi decoction group, western medicine group, and natural rehabilitation group. Xiaochengqi decoction combined with improper diet were used to establish the model of spleen deficiency rats. The gastric tissues were taken out after 4 weeks of drug intragastric intervention. The gastric mucosal injury index was calculated by the Guth method and the expression levels of TNF-α and IL-6 were detected by ELISA method, and analyzed the gastric mucosal injury. Results: Compared with the blank control group, the levels of IL-6 and TNF-α and the gastric mucosal injury in rats after modeling were obvious higher. Compared with the spleen deficiency model group and the natural rehabilitation group, the levels of IL-6 and TNF- α in Sijunzi decoction group and western medicine group were obvious decreased, the gastric mucosal injury was significantly reduced, the differences were statistically significant (P＜0.01), but there was no significant difference between the two drug treatment groups (P＞0.05). Conclusion: Sijunzi decoction can significantly improve the gastric mucosal ulcer injury in rats with spleen deficiency syndrome, which may repair gastric mucosa by downregulating the expression of TNF-α and IL-6 to improve the spleen deficiency. |
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