| 赖燕清.基于网络药理学探讨三七治疗骨关节炎的有效活性成分及潜在作用机制[J].实用中西医结合临床,2021,21(10):156-159 |
| 基于网络药理学探讨三七治疗骨关节炎的有效活性成分及潜在作用机制 |
| Investigation on the Effective Active Ingredients and Potential Mechanism of Panax Notoginseng in the Treatment of Osteoarthritis Based on Network Pharmacology |
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| DOI： |
| 中文关键词: 骨关节炎 三七 网络药理学 有效活性成分 分子机制 |
| 英文关键词: Osteoarthritis Panax notoginseng Network pharmacology Effective active ingredients Potential mechanism |
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| 中文摘要: |
| 目的：基于网络药理学探讨三七治疗骨关节炎的主要有效物质及其潜在作用机制。方法：采用中药系统药理学数据库与分析平台、草药成分指标数据库以及PubChem数据库检索三七的有效活性成分及靶点；并通过治疗靶点数据库和GeneCards数据库构建OA靶点，再使用DAVID数据库对三七-OA靶点进行GO和KEGG通路富集分析，使用STRING构建蛋白-蛋白网络，最后使用Cytoscape 3.7.1软件构建三七-活性成分-靶点-疾病网络图，并根据自由度找出关键基因。结果：从三七中共筛选出8个有效成分，与骨关节炎共同作用靶点有88个，其中最主要的三个活性成分为β-谷甾醇、人参皂苷Rh2及槲皮素。GO富集结果表明三七治疗骨关节炎主要涉及炎症反应、衰老、缺氧等生物学过程；KEGG分析显示三七治疗骨关节炎主要通过肿瘤坏死因子信号通路、衰老及Toll样受体相关通路等起作用；前三位关键基因为白细胞介素-6、肿瘤坏死因子、AKT1。结论：本研究采用网络药理学方法，初步揭示了三七治疗OA的主要有效成分、治疗靶点及相关信号通路，为进一步研究其作用机制提供参考。 |
| 英文摘要: |
| Objective: To investigate the effective active ingredients and potential mechanism of Panax notoginseng in the treatment of osteoarthritis based on network pharmacology. Methods: The effective active ingredients and targets of Panax notoginseng were retrieved by TCMSP, HIT and PubChem databases. OA target was constructed by TTD and GeneCards database, then the GO and KEGG pathway enrichment analysis about Panax notoginseng-OA target points were carried out by using DAVID database. The protein-protein network was constructed by using STRING. Finally, the network diagram of Panax notoginseng-active ingredient-target-disease were constructed by using Cytoscape 3.7.1 software, and the key genes were identified according to the degree of freedom. Results: A total of 8 active ingredients were screened from Panax notoginseng, and 88 targets were found to interact with osteoarthritis, among them, the three most important active ingredients were β-Sitosterol, ginsenoside Rh2 and quercetin. The results of GO enrichment showed that the treatment of osteoarthritis by Panax notoginseng mainly involved biological processes such as inflammation, aging, and hypoxia; KEGG analysis showed that the treatment of osteoarthritis is mainly through tumor necrosis factor signaling pathways, aging and Toll-like receptor-related pathways; and the top three hub genes were Interleukin-6, tumor necrosis factor, AKT1. Conclusion: The study use the method of network pharmacology to initially reveal the main active ingredients, therapeutic targets and related signal pathways of Panax notoginseng for osteoarthritis, which provides a reference for further study of its mechanism of action. |
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