龙璠 张湘.环介导等温扩增技术、痰涂片镜检法及固体培养法诊断肺结核病的临床价值[J].实用中西医结合临床,2021,21(6):9-10,99 |
环介导等温扩增技术、痰涂片镜检法及固体培养法诊断肺结核病的临床价值 |
The Clinical Value of Loop-mediated Isothermal Amplification Technique, Sputum Smear Microscopyand Solid Culture Method in Diagnosis of Pulmonary Tuberculosis |
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DOI: |
中文关键词: 肺结核病 环介导等温扩增技术 痰涂片镜检法 固体培养法 |
英文关键词: Pulmonary%tuberculosis %Loop-mediated%isothermal%amplification%technique %Sputum%smear%microscopy %Solid%culture%
method |
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中文摘要: |
目的:探讨环介导等温扩增技术、痰涂片镜检法及固体培养法诊断肺结核病的临床价值。 方法:选择 2019 年 4 月 ~2020
年 4 月期间就诊的 182 例初诊有肺结核可疑症状患者作为研究对象,所有入选者均提供 1 份痰标本,分别采用痰涂片镜检法、固体培
养法及环介导等温扩增技术检测。 分析环介导等温扩增技术、痰涂片镜检法及固体培养法在肺结核病中的诊断效能,计算灵敏度、
特异度、准确度、阳性预测值及阴性预测值。 结果:环介导等温扩增技术灵敏度、特异度、准确度、阳性预测值、阴性预测值均高于痰
涂片镜检法,其灵敏度、准确度、阴性预测值也均高于固体培养法,差异均有统计学意义( P <0.05 )。结论:肺结核病诊断中环介导等
温扩增技术诊断价值较高,利于临床提高肺结核病的诊断准确率,在结核病防治机构中具有较好的应用前景。 |
英文摘要: |
Objective:%To%explore%the%clinical%value%of%loop-mediated%isothermal%amplification%technique,%sputum%smear%microscopy%
and%solid%culture%method%in%diagnosis%of%pulmonary%tuberculosis.%Methods:%From%April%2019%to%April%2020,%182%newly%diagnosed%patients%
with%suspected%symptoms%of%pulmonary%tuberculosis%were%selected%as%the%research%objects.%All%patients%were%provided%with%a%sputum%
sample,%which%was%detected%by%loop-mediated%isothermal%amplification%technique,%sputum%smear%microscopy%and%solid%culture%method.%
Analyzed%the%diagnostic%efficacy%of%loop-mediated%isothermal%amplification%technique,%sputum%smear%microscopy%and%solid%culture%method%
in%pulmonary%tuberculosis,%and%calculated%the%sensitivity,%specificity,%accuracy,%positive%predictive%value%and%negative%predictive%value%of%
each%method.%Results:%The%sensitivity,%specificity,%accuracy,%positive%predictive%value%and%negative%predictive%value%of%loop-mediated%
isothermal% amplification% technique% were% higher% than% those% of% sputum% smear% microscopy;% and% the% sensitivity,% accuracy% and% negative%
predictive%value%were%also%higher%than%those%of%solid%culture%method,%the%differences%were%statistically%significant%( P <0.05).%Conclusion:%In%
the%diagnosis%of%tuberculosis,%loop-mediated%isothermal%amplification%technique%has%high%diagnostic%value,%which%is%conducive%to%improve%
the%accuracy%of%clinical%diagnosis%of%pulmonary%tuberculosis,%and%has%a%good%application%prospect%in%tuberculosis%prevention%and%control%
institutions. |
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