文章摘要
赖燕清.基于网络药理学探讨三七对骨关节炎的有效活性成分及潜在作用机制[J].实用中西医结合临床,2021,21(10):
基于网络药理学探讨三七对骨关节炎的有效活性成分及潜在作用机制
Discussion on the effective active ingredients and potential mechanism of Panax notoginseng for osteoarthritis based on network pharmacology
投稿时间:2021-03-25  修订日期:2021-04-13
DOI:
中文关键词: 三七  骨关节炎  网络药理学  有效活性成分  分子机制
英文关键词: Panax notoginseng  osteoarthritis  network pharmacology  effective active ingredients  molecular mechanism.
基金项目:
作者单位E-mail
赖燕清 福建医科大学附属龙岩第一医院 福建龙岩 364000 252916099@qq.com 
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中文摘要:
      基于网络药理学技术探讨三七治疗骨关节炎(Osteoarthritiis,OA)的主要有效物质,并探讨三七治疗OA的潜在作用机制。方法:使用TCMSP、HIT以及PubChem数据库检索三七的有效活性成分及靶点;使用TTD和GeneCards数据库构建OA靶点。使用DAVID数据库对三七-OA靶点进行GO和KEGG通路富集分析,使用STRING构建蛋白蛋白网络(PPI),使用Cytoscape3.7.1软件构建三七-活性成分-靶点-疾病网络图,并根据自由度找出关键基因。结果:从三七中共筛选出8个有效成分,与OA共同作用靶点88个,其中最主要的三个活性成分为β-谷甾醇、人参皂苷Rh2以及槲皮素。GO富集结果表明三七治疗OA主要涉及炎症反应、衰老、缺氧等生物学过程;KEGG分析显示三七治疗OA主要通过TNF信号通路、衰老以及Toll样受体相关通路等;前3位关键基因分别为IL6、TNF、AKT1。结论:本研究利用网络药理学的方法,初步揭示了三七对于OA的主要活性成分、治疗靶点及相关信号通路,为进一步深入研究其作用机制提供了参考。
英文摘要:
      To explore the main effective substances and potential mechanism of Panax notoginseng in the treatment of osteoarthritis (OA) based on the network pharmacology technology. Methods: Use TCMSP, HIT and PubChem databases to search for the effective active ingredients and targets of Panax notoginseng; use TTD and GeneCards databases to construct OA target genes. Use the DAVID database to analyze the GO and KEGG pathway enrichment analysis of the Panax notoginseng-OA targets, use STRING to construct the protein-protein network (PPI), and use the Cytoscape 3.7.1 software to construct the Panax notoginseng-active ingredient-target-disease network diagram, and identify the hub genes according to the degree of freedom. Results: A total of 8 active ingredients were screened out from Panax Notoginseng, 88 targets that interacted with OA, and the most important active ingredients were β-sitosterol, ginsenoside Rh2 and quercetin. The results of GO enrichment showed that the treatment of OA by Panax notoginseng mainly involves biological processes such as inflammation, aging, and hypoxia; KEGG analysis shows that the treatment of OA is mainly through TNF signaling pathways, aging and Toll-like receptor-related pathways; and the top 3 hub genes were IL6, TNF, and AKT1. Conclusion: This study uses the method of network pharmacology to initially reveal the main active ingredients, therapeutic targets and related signal pathways of Panax notoginseng for OA, which provides a reference for further study of its mechanism of action.
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