| 黄斌,胡燕珍,陈乐,吴先昊,王亚敏,李雪.基于UPLC-Q-TOF-MS技术分析乌蕨中的化学成分[J].实用中西医结合临床,2021,21(9): |
| 基于UPLC-Q-TOF-MS技术分析乌蕨中的化学成分 |
| Analysis of chemical constituents in Odontosoria chinensis based on UPLC-Q-TOF-MS |
| 投稿时间：2021-02-03 修订日期：2021-02-26 |
| DOI： |
| 中文关键词: 乌蕨 UPLC-Q-TOF-MS/MS 化学成分 黄酮 有机酸 |
| 英文关键词: Odontosoria chinensis UPLC-Q-TOF-MS/MS chemical constituents flavonoids organic acids |
| 基金项目: |
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| 中文摘要: |
| 目的：快速识别乌蕨中化学成分。方法：利用UPLC-Q-TOF-MS/MS技术对乌蕨药材进行分析,采用Waters C18色谱柱,流动相为乙腈-0.2%甲酸溶液,流速为0.25 ml/min,柱温40℃；采用正、负离子模式进行检测；利用PeakView 1.2软件对所得的成分进行分析,整理其母离子,及二级碎片,通过与对照品、相关文献比对,分析其裂解途径,推断其化合物结构。结果：共推断出乌蕨中32种化合物,包括15个黄酮类化合物,15个有机酸类化合物,2个香豆素类成分,其中原儿茶酸葡萄糖苷、咖啡酰葡萄糖苷首次从乌蕨中发现。结论：从液质结果及相关文献表明,乌蕨中以有机酸、黄酮及其苷类成分为主。本研究可为乌蕨的药效物质基础研究提供参考依据。 |
| 英文摘要: |
| Objective Rapid identification of chemical constituents from Odontosoria chinensis. Methods UPLC-Q-TOF-MS/MS technology was used to analyze Odontosoria chinensis, The determination was performed on Waters C18 column with a mobile phase using water containing 0.2％formic acid and acetonitrile.The flow rate was 0.3 ml / min and the column temperature was 40 ℃. The positive and negative ion mode was used to detect. The components were analyzed by peakview 1.2 software, and the parent ions and secondary fragments were sorted out,By comparing with the reference chromatogram and relevant literature, finally, the structure of the compound was deduced. |
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