| 郭银雪,葛平玉.肾茶总黄酮调节肾缺血再灌注损伤大鼠肾小管上皮细胞凋亡的作用和机制[J].实用中西医结合临床,2020,20(1): |
| 肾茶总黄酮调节肾缺血再灌注损伤大鼠肾小管上皮细胞凋亡的作用和机制 |
| Effect and mechanism of total flavonoids from kidney tea on apoptosis of renal tubular epithelial cells in rats with renal ischemia reperfusion injuryGUO Yin-xue,GE Ping-yu(The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China) |
| 投稿时间：2019-07-08 修订日期：2019-07-28 |
| DOI： |
| 中文关键词: 肾茶总黄酮 肾小管上皮细胞凋亡 超氧化物歧化酶 一氧化氮合酶 丙二醛 |
| 英文关键词: Total Flavonoids of Clerodendranthus spicatus Epithelial Cells Apoptosis of Renal Tubule SOD NOS MDA |
| 基金项目:贵州省科技计划项目(黔科合LH字[2014]7346号) |
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| 中文摘要: |
| 目的 探究肾茶总黄酮调节实验大鼠缺血再灌注损伤后肾小管上皮细胞凋亡的作用和机制。方法 60只雄性实验SD大鼠，信封法随机编码分为4组（n=15只），对照组、模型组、肾茶总黄酮小剂量治疗组、肾茶总黄酮大剂量治疗组。模型组、肾茶总黄酮小剂量治疗组和肾茶总黄酮大剂量治疗组均采用手术摘除实验大鼠右侧肾脏，持续阻断左肾动脉1h后恢复灌注的方法建立急性肾缺血再灌注损伤模型，通过酶偶联法测定模型大鼠血肌酐（Cr）、尿素氮（BUN）的变化并比较，确认模型建立成功；对照组、模型组以0.9%氯化钠溶液灌胃，治疗组以肾茶总黄酮溶液灌胃（高剂量400mg/Kg、小剂量100mg/Kg），治疗4天；TUNEL法检测大鼠肾小管上皮细胞凋亡指数（AI）；肾组织匀浆后，黄嘌呤氧化酶法测定SOD，化学比色法测定NOS和硫代巴比妥酸法测定MDA含量。结果 模型组Cr（94.76±13.83）、BUN（25.34±2.46），与对照组Cr（55.41±9.83）、BUN（13.84±1.53）比较显著升高（P＜0.05），显示造模成功；经肾茶总黄酮大剂量治疗后Cr（68.64±11.21）、BUN（15.13±1.44）、AI（ 4.37±4.29）、NOS（11.57±2.25）和MDA（11.32±0.91）显著降低（P＜0.05），SOD（ 29.84±5.58）显著升高，差异有统计学意义（P＜0.05）。结论 肾茶总黄酮能够减轻肾缺血再灌注损伤大鼠的急性肾损伤（AKI），这种作用可能通过下调肾小管上皮细胞的凋亡来实现，其机制可能是减少大鼠肾组织内自由基生成，增强其抗自由基损伤的能力。 |
| 英文摘要: |
| Objective To explore the effect and mechanism of total flavonoids of kidney tea on the apoptosis of renal tubular epithelial cells after ischemia-reperfusion injury in experimental rats.Methods Sixty male Sprague-Dawley rats were randomly divided into 4 groups (n=15), control group, model group, kidney tea total flavonoids low-dose treatment group, and kidney tea total flavonoids.The Model group, kidney tea total flavonoids low-dose treatment group and kidney tea total flavonoids in the high-dose treatment group were surgically removed from the right kidney of the experimental rats, and the left renal artery was continuously blocked for 1 h after reperfusion. Acute renal ischemia-reperfusion was established. In the injury model, the changes of serum creatinine (Cr) and urea nitrogen (BUN) in the model rats were determined by enzyme coupling method, and the model was established successfully. The control group and the model group were intragastrically administered with 0.9% sodium chloride solution. The total flavonoid solution of kidney tea was intragastrically administered (high dose 400mg/Kg, low dose 100mg/Kg) for 4 days; TUNEL method was used to detect the apoptosis index (AI) of rat renal tubular epithelial cells; after renal homogenate, jaundice SOD was determined by oxidase method, and MDA content was determined by chemical colorimetric method for determination of NOS and thiobarbituric acid. Results The model group Cr (94.76±13.83) and BUN (25.34±2.46) were significantly higher than the control group Cr (55.41±9.83) and BUN (13.84±1.53) (P<0.05), indicating successful modeling; After treatment with high dose of total flavonoids, Cr (68.64±11.21), BUN (15.13±1.44), AI (4.37±4.29), NOS (11.57±2.25) and MDA (11.32±0.91) were significantly decreased (P<0.05), SOD ( 29.84±5.58) was significantly elevated, and the difference was statistically significant (P<0.05). Conclusion Total flavonoids from Clerodendranthus spicatus can inhibit the apoptosis of renal tubular epithelial cells in rats with acute renal ischemia-reperfusion injury. The mechanism may be to reduce the free radical formation in rat kidney tissue and improve its anti-free radical ability. |
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