| 赵锦燕,尚海霞,曾建伟,兰炜兰,洪振丰.微小RNA在肝癌耐药Bel-7402/5-FU细胞中的表达分析[J].实用中西医结合临床,2019,19(9): |
| 微小RNA在肝癌耐药Bel-7402/5-FU细胞中的表达分析 |
| Analysis of miRNA in the hepatocellular carcinoma resistant cells Bel-7402/5-FU |
| 投稿时间：2019-06-04 修订日期：2019-06-27 |
| DOI： |
| 中文关键词: 肝癌 多药耐药 微小RNA |
| 英文关键词: hepatocellular carcinoma, multidrug resistance, microRNA |
| 基金项目:国家自然科学基金资助( 81303125 )；福建省自然科学基金资助(2017J01845) |
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| 中文摘要: |
| 目的 通过检测肝癌亲本细胞Bel-7402及其5-FU耐药细胞Bel-7402/5-FU中微小RNA(microRNA,miRNA)的表达差异,探讨miRNA在肝癌细胞多药耐药中的作用。方法 体外培养Bel-7402及其耐药株Bel-7402/5-FU细胞,MTT法检测Bel-7402/5-FU细胞对不同化疗药物的敏感性,miRNA表达芯片检测Bel-7402细胞和Bel-7402/5-FU细胞miRNA的差异表达,qPCR验证差异表达的miRNA。结果 Bel-7402/5-FU对不同的化疗药物均具有耐药性；miRNA芯片检测结果发现,耐药株Bel-7402/5-FU与亲本Bel-7402细胞中,差异表达的miRNA有29个,其中上调14个,下调15个；qPCR验证结果发现,和亲本Bel-7402细胞比较,耐药株Bel-7402/5-FU细胞中miR-10a,-371a-3p,-371a-5p,-372,-373-3p,-146-5p,6723-5p显著下调,miR-4497,-3148显著上调。结论：肝癌多药耐药的发生与miRNA表达异常有关。 |
| 英文摘要: |
| s: Objective To determined the different experssion of miRNA in the parental cells Bel-7402 and its resistant cells Bel-7402/5-FU and investigated the effect of miRNA in the multidrug resistance (MDR) of hepatocellular carcinoma (HCC) . Methods MTT assays were used to detect the sensitivity to different chemotherapy drug of Bel-7402/5-FU. The miRNA array was used to detect the different expression between Bel-7402 and Bel-7402/5-FU. qPCR assay was used to verified the different miRNA. Results Bel-7402/5-FU cells were resistant to diferent chemotherapy drug. In miRNA array analysis, Twenty-nine of miRNAs were changed, and fourteen of miRNAs were up-regulated, fifteen were down-regulated. The verified results of qPCR showed that miR-10a,-371a-3p,-371a-5p,-372,-373-3p,-146-5p,6723-5p were down-regulated,miR-4497,-3148 were up-regulated in BEL-7402/5-FU cells. Conclusions The diferent expression of miRNA was related to the MDR of HCC. |
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