| 曹治云，余旋，魏丽慧，曾建伟，赵锦燕，黄彬，林久茂.清解扶正颗粒体外抑制血管新生的作用机制研究[J].实用中西医结合临床,2019,19(1):4-6,160 |
| 清解扶正颗粒体外抑制血管新生的作用机制研究 |
| The Antiangiogenic Effect of the Qingjiefuzheng Granules on HUVEC in Vitro |
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| DOI： |
| 中文关键词: 清解扶正颗粒 人脐静脉内皮细胞 血管新生 血管内皮生长因子 基质金属蛋白酶 |
| 英文关键词: The Qingjiefuzheng granules Human Umbilical Vein Endothelial Cells Angiogenesis Vascular endothelial growth factor Matrix metalloproteinase |
| 基金项目:国家自然科学基金资助项目（编号：81873166）；福建省自然科学基金项目（编号：2017J01542）；福建省中医药科研课题（编号：2017FJZYZY203）；福建省卫生计生中青年骨干人才培养项目（编号：2016-ZQN-67） |
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| 中文摘要: |
| 目的：探讨清解扶正颗粒（Qingjiefuzheng Granules，QFG）对体外人脐静脉内皮细胞（Human Umbilical Vein Endothelial Cells，HUVEC）新生的影响及可能的作用机制。方法：以HUVEC为研究对象，给予不同浓度QFG后观察细胞形态变化，MTT检测细胞活力，划痕实验检测细胞迁移能力，管腔形成实验检测细胞成血管能力，蛋白免疫印迹检测促血管新生因子、血管内皮生长因子A、血管内皮生长因子受体2、基质金属蛋白酶2及基质金属蛋白酶9的蛋白表达。结果：QFG对HUVEC形态与细胞生长没有明显影响，但可显著抑制HUVEC的活力、迁移及体外成血管能力；QFG显著抑制血管内皮生长因子A、基质金属蛋白酶2、基质金属蛋白酶9等蛋白表达。结论：QFG在体外具有抑制血管新生的作用，其作用机制与其抑制血管内皮生长因子A、基质金属蛋白酶2及基质金属蛋白酶的表达有关。 |
| 英文摘要: |
| Objective: To investigate the effect of the Qingjiefuzheng granules on the regenaration of HUVEC in vitro and the possible antiangiogenic mechanism. Method: HUVEC was used as the research object, and the morphological changes were observed after different concentrations of QFG. The cell viability was detected by MTT, the cell migration ability was detected by scratch test, and the angiogenic ability of cells was detected by lumen formation test, and the protein expression of pro-angiogenic factor, VEGFR-2, VEGF-A, MMP-2, MMP-9 in treated HUVEC was evaluated by western blot. Results: QFG had no significant effect on HUVEC morphology and cell growth, but significantly inhibited the HUVEC viability, migration ability and in vitro angiogenic ability. Furtherly, QFG significantly inhibited the expression of VEGF-A, MMP-2, MMP-9. Conclusion: QFG has an inhibitory effect on angiogenesis of HUVEC in vitro, and its mechanism is related to its inhibition of expression of VEFG-A, MMP-2 and MMP-9. |
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