| 赵锦燕,张毓宸,万芸,曾建伟,洪振丰.片仔癀肝宝对慢性肝损伤凋亡的影响[J].实用中西医结合临床,2017,17(1): |
| 片仔癀肝宝对慢性肝损伤凋亡的影响 |
| Effect of Pien Tze Huang Gan Bao on the apoptosis of chronic liver Injury |
| 投稿时间：2016-12-19 修订日期：2016-12-30 |
| DOI： |
| 中文关键词: 片仔癀肝宝 肝损伤 凋亡 |
| 英文关键词: |
| 基金项目:陈可冀中西医结合发展基金(CKJ2013015) |
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| 中文摘要: |
| 目的 探讨片仔癀肝宝(肝宝)对CCl4诱导大鼠慢性肝损伤凋亡的影响。方法 60只SD大鼠随机分为正常组、模型组、西利宾胺组、GB低剂量组、GB中剂量组、GB高剂量组,每组10只。除正常组外,SD大鼠腹腔注射50% CCl4橄榄油溶液1 mL/kg,2次/周,连续8周。于造模第四周开始给药,设正常对照组(生理盐水)、模型组(生理盐水)、阳性药对照组(西利宾胺,50 mg/kg),肝宝低剂量组(150 mg/kg)、肝宝中剂量组(300 mg/kg)、肝宝高剂量组(600 mg/kg),1次/d,连续4周。于末次给药24h后, HE染色观察肝宝对肝组织病理学的影响,TUNEL法观察肝组织凋亡情况,比色法检测Caspase-3和Caspase-9的变化,western blot检测肝宝对Bax和Bcl-2蛋白表达的影响。 结果 HE染色发现,各用药组均可不同程度缓解CCl4诱导的肝损伤；肝宝抑制肝细胞凋亡；降低Caspase-3和Caspase-9的活力；下调Bax表达,上调Bcl-2表达。结论 片仔癀肝宝可以抑制CCl4诱导的肝细胞凋亡。 |
| 英文摘要: |
| Objective To explore the effect of Pien Tze Huang Gan Bao (GB) on the apotosis of chronic liver injury induced by CCl4 in rats. Methods Sixty Sprague–Dawley (SD) rats were randomly divided into control, model, silymarin, GB-low, GB-middle and GB-high groups, ten rats per group. Except the control was received olive oil, the rest groups were intraperitoneally injected with CCl4 1 ml/kg, twice/week for constitutively 8 weeks. At the fourth week, the model and the control were orally administration physiological saline (PS), while the silymarin (50 mg/kg), GB-Low (150 mg/kg), GB-middle (300 mg/kg), GB-high (600 mg/kg) were orally administrated in treatment group respectively for 4 weeks. Twenty-four hours after the last treatment, HE staining was used to observe the histopathological change in liver; The apoptosis was determined by TUNEL assay; Caspase-3 and Caspase-9 were detected; The protein expression of Bax and Bcl-2 were determined by western blot. Results The results from HE staining found that all treatment could alleviate the injuried liver tissues differently; GB could inhibit the apotosis and decrease the activity of Caspase-3 and Caspase-9; Furthermore, GB down-regulated the expression of Bax and up-regulated Bcl-2. Conclusion GB Could inhibit the apotosis induced by CCl4. |
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