| 赵锦燕,刘丽雅,张毓宸,冯健愉,曾建伟,洪振丰.小柴胡汤逆转人肝癌细胞BEL-7402／5-FU 的多药耐药作用[J].实用中西医结合临床,2016,16(9): |
| 小柴胡汤逆转人肝癌细胞BEL-7402／5-FU 的多药耐药作用 |
| Xiaochaihu Tang reverses multidrug resistance in resistant human hepatocellular carcinoma cell line BEL-7402/5-FU |
| 投稿时间：2016-09-19 修订日期：2016-09-30 |
| DOI： |
| 中文关键词: 小柴胡汤 肝癌 多药耐药 逆转 |
| 英文关键词: Xiaochaihu Tang hepatocellular carcinoma multi-drug resistance reverses |
| 基金项目:国家自然科学(81303125) |
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| 中文摘要: |
| 目的 研究小柴胡汤对BEL-7402／5-FU 细胞多药耐药的逆转作用,并初步探讨其作用机制。方法 四甲基偶氮唑蓝(MTT)法检测不同化疗药物以及小柴胡汤对BEL-7402／5-FU 细胞的半数抑制浓度 (IC50),计算耐药指数和小柴胡汤的逆转倍数；流式细胞仪检测细胞内阿霉素的荧光强度；RT-PCR检测mdr1基因表达；western blot检测p-gp蛋白表达。 结果 BEL-7402／5-FU耐药株对5-FU和阿霉素顺铂均具有耐药性,耐药指数分别是6501.99 和31.73；0.5mg/ml小柴胡汤和5-FU、阿霉素联合应用,其逆转倍数分别是1.66,1.50；1.0mg/ml小柴胡汤和5-FU、阿霉素联合应用, 其逆转倍数分别是3.44,5.58；小柴胡汤增强阿霉素的荧光强度；小柴胡汤降低mdr1基因表达和p-gp蛋白表达。 |
| 英文摘要: |
| Objective To determine the reversal effect of Xiaochaihu Tang (XCHT) on the resistant human hepatocellular carcinoma cell line BEL-7402/5-FU and explore its mechanism．Methods The IC50 of BEL-7402/5-FU cells was examined by MTT and calculated the Resistance index (RI) and the reversal folds (RF)of XCHT. Intracellular ADM concentration was determined by Flow cytometry. RT-PCR and western blot was used to detected the expression of mdr1 and p-gp,respectively. Results BEL-7402/5-FU showed resistance to variety of chemotherapeutic drugs,the Resistance index ( RI) of 5-FU or Adriamycin (ADM) was 6501.99 or 31.73.XCHT (0.5mg/ml or 1.0mg/ml) combined with 5-FU and ADM, the reversal fold was 1.66 and 1.50 or 3.44 and 5.58. XCHT increased the intracellular concentration of ADM. XCHT down-regulated the mdr1 and p-pg expression. Conclusion XCHT could inhibit the expression of p-gp coded by mdr1 gene, and increase the intracellular concentration of chemotherapeutic drugs, so as to reverse the multidrug resistance of HCC cells. |
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