| 周美英,魏林珍,王海琳,张爱笠,甄洁玉.苦参碱对宫颈癌SiHa细胞凋亡的调节机制研究[J].实用中西医结合临床,2016,16(8): |
| 苦参碱对宫颈癌SiHa细胞凋亡的调节机制研究 |
| Effects of Matrine on Apoptosis of Human Cervical Cancer Cell Line SiHa and Its Mechanism |
| 投稿时间：2016-06-23 修订日期：2016-07-24 |
| DOI： |
| 中文关键词: 苦参碱 宫颈癌 细胞增殖 细胞凋亡 Z-DEVD-FMK,caspase-3 |
| 英文关键词: matrine cancer breed apoptosis Z-DEVD-FMK, Caspase-3 |
| 基金项目:] 国家自然科学资金(H1621);甘肃省自然基金(1208RJZA109) |
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| 中文摘要: |
| 目的 观察苦参碱体外诱导宫颈癌SiHa细胞凋亡效应并对其可能的作用机制进行初步探讨。方法 将不同浓度(0、0.25、0.5、1.00、2.00 mg.mL -1)的苦参碱分别作用于体外培养的宫颈癌细胞,在不同时间点(24、48、72h),采用MTT法检测细胞的增殖情况；采用Annexin-Ⅴ和PI双染法在流式细胞仪上检测细胞的凋亡率；采用Western blot 检测细胞中凋亡相关蛋白Caspase- 3的表达。结果 各组苦参碱对SiHa细胞的增殖均有抑制作用,MTT和流式细胞结果显示,不同浓度的苦参碱对细胞生长抑制率与对照组相比存在明显差异(P均<0. 05 ),呈剂量效应正相关及时间效应正相关。加入Caspase-3抑制剂Z-DEVD-FMK后,各组细胞凋亡率与只加苦参碱组相比凋亡率下降。Western blot 检测结果显示Caspase- 3蛋白水平随苦参碱浓度的提高而增加(P<0. 05)。结论 苦参碱可诱导宫颈癌SiHa细胞的增殖,促进其凋亡,其作用机制可能与提高Caspase-3活性有关。 |
| 英文摘要: |
| AIM To invest the role of matrine(Mat) induced apoptosis of human cervical cancer Siha cells and its mechanism. METHODS Siha cells were exposed to the concentrations of matrine 0, 0. 25, 0.5, 1. 00 and 2. 00 mg.mL -1 for 24,48,72h. Cell proliferation was detected by MTT assay. Apoptosis was determined by double stained assay with Annexin-Ⅴand PI. Whether the apoptotic effect of matrine on cervical cancer cell is partially blocked by Z-DEVD-FMK,a caspase-3 inhibitor, was evaluated by flow cytometry. Caspase-3 protein level was determined with western blot assay. RESULTS After Siha cells were cocultured with 0, 0.5, 1. 0, 2. 0mg.mL -1 matrine for 24, 48, 72h in vitro, the cell proliferation were inhibited in time and dose dependent manner. When the Siha cells were culture for 24,48,72h by matrine 0.5,1.0, 2.0mg.mL -1,the total apoptosis rate was increased ,which had ststistical significance compared with control group (P all <0. 05 ), After treatment with matrine (0.5,1.0,2.0 mg.mL -1) in the presence ( ) or absence (--) of Z-DEVD-FMK (25 umol.L -1) for 48 h,Siha cells were partially blocked by caspase-3 inhibitor Z-DEVD-FMK. The protein expression level of Caspase-3 increased after Siha cells were treated by matrine 0,0.5,1.0, 2.0 mg.mL -1 with a concentration effect relationship(P<0. 05) . CONCLUSION Matrine can inhibit the proliferation and induce the apoptosis of Siha cells in vitro, one of its possible mechanisms is that matrine increasing caspase-3 activity. |
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