| 陈达鑫,林珊,赵锦燕,曾建伟,洪振丰.复方片仔癀肝宝对酒精诱导的慢性肝损伤氧化应激的保护作用研究[J].实用中西医结合临床,2016,16(7): |
| 复方片仔癀肝宝对酒精诱导的慢性肝损伤氧化应激的保护作用研究 |
| Complex Prescription Pien Tze Huang Ganbao protects against alcohol-induced chronic liver injury caused by oxidative stress |
| 投稿时间：2016-06-08 修订日期：2016-06-09 |
| DOI： |
| 中文关键词: 片仔癀肝宝 酒精性肝损伤 氧化应激 |
| 英文关键词: Pien Tze Huang Ganbao Alcoholic liver disease Oxidative stress |
| 基金项目:福建省科技厅项目(2010YZ0001-1) |
|
| 摘要点击次数: 843 |
| 全文下载次数: 542 |
| 中文摘要: |
| 目的 探讨复方片仔癀肝宝对酒精性肝病(aleoholie liver disease,ALD)模型大鼠氧化应激的保护作用。方法 60只SPF级SD大鼠,随机分为空白对照组、模型组、阳性药对照组、肝宝低、中、高剂量组,每组10只。除空白对照组外其余各组用酒精联合高脂饲料喂养2周,造ALD模型,连续给药4周后取材。全自动生化仪检测血清中谷丙转氨酶(ALT),谷草转氨酶(AST),碱性磷酸酶(ALP),乳酸脱氢酶(LDH)的含量变化；检测肝组织中超氧化物歧化酶(SOD),丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)的表达；HE染色,观察肝组织病理变化。结果 模型组血清中ALT、AST、ALP、LDH的活性较正常对照组均有明显的上升,且肝组织中MDA水平明显上升,SOD, GSH-Px水平明显下降(P<0.01)。与模型组相比,肝宝各组ALT、AST、ALP、LDH的活性及MDA水平明显降低,而SOD、 GSH-Px水平明显上升(P <0.05)。HE染色结果显示复方片仔癀肝宝不同剂量组对肝组织脂肪变性、肝内脂类聚集有明显的改善作用,并且存在剂量效应 |
| 英文摘要: |
| Objective Determine the protective effect of Complex Prescription Pien Tze Huang Ganbao (GB) in alcoholic liver disease (ALD) using a rat model of oxidative stress. Methods 60 SPF SD rats were randomly divided into control group, model group, positive control group GB low, medium and high dose groups (n=10 for each group). All groups were fed an alcohol high fat diet for two weeks, as a model for ALD, and rats were sacrificed following 4 weeks of feeding. The level of expression of automatic biochemical analyzer serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) and liver tissue superoxide dismutase (SOD) were detected. HE staining of changes in liver tissue pathology were determined, as well as malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) expression. Results The ALD model group had significantly increased levels of ALT, AST, ALP and LDH activity compared to the control group. In addition, liver tissue levels of MDA was significantly increased, whereas levels of SOD and GSH-Px were significantly decreased (P <0.01). Compared with the ALD model group, GB treated group had significantly decreased expression of ALT, AST, ALP, LDH activity and MDA levels, whereas SOD and GSH-Px levels were significantly increased (P <0.05). HE staining showed that different doses of GB resulted in significant improvements to hepatic steatosis and hepatic lipid accumulation, with a dose-response relationship. Conclusion Complex Prescription Pien Tze Huang Ganbao can significantly reduce fat and alcohol-induced liver damage, which may be beneficial in improving the antioxidant capacity of liver tissue. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
