| 罗文艳,段和祥.蒌蒿乙酸乙酯部位体外抗乙型肝炎病毒的实验研究[J].实用中西医结合临床,2016,16(9): |
| 蒌蒿乙酸乙酯部位体外抗乙型肝炎病毒的实验研究 |
| Experimental study on Artemisia selengensis fration anti-hepatitis B Virus in vitro |
| 投稿时间：2016-06-02 修订日期：2016-06-30 |
| DOI： |
| 中文关键词: 蒌蒿提取物 乙型肝炎病毒 乙型肝炎病毒表面抗原 乙型肝炎病毒e抗原 |
| 英文关键词: Artemisia selengensis fraction hepatitis B Virus HBsAg HBeAg |
| 基金项目:江西省卫生厅中医指导性计划(2013B014);江西省科技厅青年科学基金资助项目(20122BAB215044) |
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| 中文摘要: |
| 目的：研究蒌蒿乙酸乙酯部位体外抗乙型肝炎病毒(HBV)的作用。方法：用95%乙醇提取蒌蒿药材后用乙酸乙酯萃取,并作用于HBV-DNA转染的HepG2.2.15细胞。采用MTT法检测乙酸乙酯部位对 HepG 2.2.15细胞的半数抑制浓度(IC50),用ELISA法观察乙酸乙酯部位对HepG2 2.2.15细胞分泌HBsAg和HBeAg表达的抑制作用。结果：蒌蒿乙酸乙酯部位对HepG2.2.15细胞有一定的细胞抑制作用,对HepG2.2.15细胞分泌 HBsAg和HBeAg表达有不同程度的抗原抑制作用。结论：蒌蒿乙酸乙酯部位体外培养细胞实验证实其有较强的抗乙型肝炎病毒作用。 |
| 英文摘要: |
| Objective:To study on Artemisia selengensis ethyl acetate parts anti- HBV in vitro. Methods: Artemisia selengensis were extracted with 95% ethanol eluent, then petroleum ether, chloroform, ethyl acetate extracted from the active fraction. Effect on HBV-DNA transfected HepG2.2.15 cells. MTT assay was used to detect ethyl acetate fraction inhibitory concentration 50% of HepG2.2.15 cells. ELISA method was used to investigate the suppressor effect of it on HBsAg and HBeAg in the cultured HepG 2. 2. 15 cells which were gene transferred with HBV. Results: Ethyl acetate fration of Artemisia selengensis have a certain cell inhibitory effect on HepG2.2.15 cells. HepG2.2.15 cells the secretion of HBsAg and HBeAg express different degrees of inhibition of antigen.Conclusions: The cells culture indicated that ethyl acetate fration could obvious anti-HBV in vitro . |
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