| 毛敬洁,李钻芳,林如辉,卓沛元,张颖铮,陈立典#.APP／PSl双转基因阿尔海默病小鼠的繁殖及基因型鉴定 *[J].实用中西医结合临床,2015,15(10): |
| APP／PSl双转基因阿尔海默病小鼠的繁殖及基因型鉴定 * |
| Reproduction and Genetype identification of APP/PS1 double transgenic Alzheimer’s disease mouse model |
| 投稿时间：2015-10-04 修订日期：2015-10-18 |
| DOI： |
| 中文关键词: APP/PS1双转基因AD小鼠 基因型 |
| 英文关键词: APP/PS1 double transgenic mouse of AD Genetype |
| 基金项目:福建省自然科学基金资助项目 (编号：2013J01378) |
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| 中文摘要: |
| 目的 繁殖和基因鉴定APP/PS1双转基因阿尔海默病(AD)小鼠。方法 将雄性纯合子APP/PS1双转基因AD小鼠与阴性纯合子(野生型)雌鼠进行交配。PCR鉴定APP/PS1双转基因AD传代小鼠的基因表型。结果 50只APP/PS1双转基因AD传代小鼠基因组DNA中31只小鼠基因组DNA扩增出300bp的APP条带和600bP的PS1条带。APP/PS1双转基因AD小鼠的饲养和繁殖获得成功,获得了较多的成功转入APP/PS1基因的纯合子小鼠。结论 正确饲养繁殖及利用PCR对AD模型鼠的繁殖小鼠进行基因鉴定,是有效获得APP/PS1双转基因纯合子小鼠的有效途径,为临床更好的研究认知障碍提供有效的AD动物模型。 |
| 英文摘要: |
| Objective To breed and identify the genetype of APP/PS1 double transgenic Alzheimer’s disease mouse model. Methods The APP/PS1 double transgenic mice were introduced and the genotyping of transgenic mice were detected by PCR. Results 50 in 31 mice were identified APP/PS1 double transgenic AD mouse by PCR, with about 300 bp size detected APP genome DNA, and 600 bp size detected PS1 genome DNA. The feeding and breeding were successful and more homozygote baby mice with APP/PS1 double transgenic mouse were attained. Conclusion: Appropriate methods of feeding, breeding and PCR identifying are the effective ways to acquire homozygote baby mice with APP/PS1 transgenic mouse. It provides the effective AD animal models for clinical research. |
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