黄板树根提取液对白细胞介素-18在骨性关节炎滑液中的表达影响及意义

王凤龙; 肖智青; 朱锦忠; 王爱明; 徐洲发; 张火林; 江建明

文章摘要

王凤龙肖智青朱锦忠王爱明徐洲发张火林江建明.黄板树根提取液对白细胞介素-18在骨性关节炎滑液中的表达影响及意义[J].实用中西医结合临床,2015,15(9):1-4

黄板树根提取液对白细胞介素-18在骨性关节炎滑液中的表达影响及意义

The Expression and Significance of Huangban Tree Extract for IL-18 in Synovial Fluid of Patients with Osteoarthritis

DOI：

中文关键词: 骨性关节炎黄板树根提取液白细胞介素-18 滑液

英文关键词: Osteoarthritis Huangban tree extract Interleukin 18 Synovial fluid

基金项目:基金项目：国家自然科学基金（编号：30571890）；广东省河源市社会发展科技计划项目（编号：2013054）

作者	单位
王凤龙肖智青朱锦忠王爱明徐洲发张火林江建明	广东省河源市中医院；南方医科大学附属南方医院

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中文摘要:

目的：了解使用黄板树根提取液外用熏洗对骨性关节炎（osteoarthritis，OA）患者白细胞介素-18（Interleukin 18，IL-18）在滑液中表达的影响及意义。方法：观察正常膝关节滑膜组织与OA病变滑膜组织在组织形态及病理切片的差异，对OA患者（n=30）使用黄板树根提取液熏洗治疗，分别提取治疗前后的关节滑液，采用酶联免疫吸附测定法（ELISA）定量检测熏洗治疗前后关节滑液中IL-18蛋白的表达水平，并比较其表达的差异。结果：正常滑膜组织肉眼观为淡红色或瓷白色，OA滑膜组织多为粉红色；病理切片显示：相比正常滑膜组织，OA滑膜组织出现明显的血管增生和炎细胞浸润，符合组织慢性炎症病变特征；ELISA检测治疗前OA患者滑液中IL-18的表达含量为（52.625±11.336） pg/ml，治疗后OA患者滑液中IL-18的表达含量为（22.512±2.021） pg/ml，两者之间具备统计学差异（P＜0.05）。结论：黄板树根提取液熏洗治疗可以使OA关节滑液中炎性因子IL-18表达水平下降，提示黄板树根提取液可能对OA有一定的治疗作用。

英文摘要:

Objective: To explore the expression and significance of Huangban tree extract for IL-18 in synovial fluid of patients with osteoarthritis (OA). Methods: Observed the open and pathological difference between normal synovium and OA synovium. Gathered the primary synovial fluid from OA patients (n=30), and then treated those patients with Huangban tree extract, taken the synovial fluid again after treatment, The concentrations of IL-18 were measured in human OA synovial fluid by enzyme-linked immunosorbent assay (ELISA), compared the difference. Results: The normal synovium was light red or white porcelain, while the OA synovium was pink. The pathological section of synovium showed: compared to normal synovium, the OA synovium had evident hyperplasy in vessel and infiltrating by inflammatory factors. This was the character of chronic inflammation. The average value of IL-18 in OA before treatment was (52.625±11.336) pg/ml. The average value after treatment was (22.512±2.021) pg/ml. There was a significant statistics difference between them (P＜0.05). Conclusion: After the treatment of Huangban tree extract IL-18 is lower expressed in synovial fluid of OA patients, it may play an important role in the treatment of OA.

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