汪军 姚闽.RP-HPLC法测定消痛灵中延胡索乙素含量的方法研究[J].实用中西医结合临床,2014,14(12):84-85 |
RP-HPLC法测定消痛灵中延胡索乙素含量的方法研究 |
Study on the Determination Method of Tetrahydropalmatine in Xiaotongling with RP-HPLC Method |
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DOI: |
中文关键词: 高效液相色谱法 消痛灵 延胡索乙素 含量测定 |
英文关键词: High performance liquid chromatography Xiaotongling Tetrahydropalmatine Determination |
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中文摘要: |
目的:建立消痛灵中延胡索乙素的含量测定方法。方法:采用高效液相色谱法,色谱柱:C18(250 mm×4.6 mm,5 ?滋m)(大连依利特);流动相:甲醇-0.01%磷酸溶液(三乙胺调pH=6.0,34∶66);柱温:25 ℃;检测波长:280 nm;流速:1.0 mL/min。结果:本实验研究最终建立了消痛灵中延胡索乙素的含量测定方法。结论:高效液相色谱法简单、准确、重现性好,可做为消痛灵质量控制方法之一。 |
英文摘要: |
Objective: To establish the method for the determination tetrahydropalmatine in Xiaotongling. Method: Used high performance liquid chromatography, a Dalian Yilite C18 column (Hypersil ODS2, 4.6 mm×250 mm, 5 μm) was used, the methol-0.01% phosphoric acid solution (adjusted to pH 6.0 with triethylamine, 34:66) as mobile phase, the column temperature was at 25 ℃, the detection wavelength was set at 280 nm, the flow rate was 1 mL/min. Results: The research finally established a method for determination the content of tetrahydropalmatine in Xiaotongling. Conclusions: This method is convenient, accurate and reproducible for the determination of tetrahydropalmatine in Xiaotongling. |
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