| 刘雯 张娣 宋友昕 李才堂 雷卓 刘强 罗文花.藤梨根拮抗四氯化碳致急性肝损伤作用活性部位的初步筛选[J].实用中西医结合临床,2014,14(2):86-88 |
| 藤梨根拮抗四氯化碳致急性肝损伤作用活性部位的初步筛选 |
| Initial Screening of the Tengligen Active Site with antagonism of ALI by CCl4 |
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| DOI： |
| 中文关键词: 藤梨根 活性部位 四氯化碳 急性肝损伤 |
| 英文关键词: Tengligen Active site CCl4 Acute liver injury |
| 基金项目: |
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| 中文摘要: |
| 目的：通过建立四氯化碳（CCl4）致急性肝损伤小鼠模型，测定其血清中谷丙转氨酶（ALT）的含量，对藤梨根不同提取部位抑制ALT升高的作用比较，筛选出具有拮抗肝损伤作用的提取部位。方法：取藤梨根原药材，经过乙醇提取、乙酸乙酯萃取，得乙酸乙酯提取部位；过聚酰胺柱洗脱，得不同提取部位，分为X部位、Y部位、Z部位；小鼠预防性给药2 d，以CCl4橄榄油混合物（1%）2 mL/kg腹腔注射造就小鼠化学性肝损伤模型，第3天给药后摘眼球取血，测血清中ALT活力。结果：乙酸乙酯部位、X部位、Y部位均具有拮抗CCl4致急性肝损伤小鼠血清ALT升高的作用，其中X部位在一定剂量范围内具有量效关系。结论：含有黄酮类化合物的藤梨根提取X部位，具有拮抗CCl4致急性肝损伤的作用，且在一定剂量范围内具有量效关系。 |
| 英文摘要: |
| Objective: Through the establishment of carbon tetrachloride (CCl4) caused by acute liver injury (ALI) model mice, to determine the serum levels of alanine transaminase (ALT) in the Tengligen different extraction parts compared to inhibit the action of the ALT elevations, select the extraction of liver damage parts have antagonism. Method: Take the Tengligen after ethanol extract and ethyl acetate extraction, ethyl acetate extracted parts; Polyamide column elution, divided into parts X, Y, Z position; Preventive medication 2 d in mice, by CCl4 olive oil mixture (1%) by intraperitoneal injection of 2 mL/kg chemical liver injury in mice model, 3 d after measured ALT activity in serum. Results: The ethyl acetate parts, and the X, Y parts have antagonism CCl4 induced acute liver injury in mice serum ALT elevations, where X parts has concentration-response relationship in certain dose range. Conclusion: Tengligen extract X parts which contain flavonoids, has antagonism CCl4 induced acute liver injury, and to a certain dose range has the concentration-response relationship. |
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