| 肖智青 张雄辉 王凤龙 朱锦忠 王爱明 江建明 肖军.黄板树根提取液对骨性关节炎作用的实验研究[J].实用中西医结合临床,2012,(2):1-3 |
| 黄板树根提取液对骨性关节炎作用的实验研究 |
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| DOI： |
| 中文关键词: 骨性关节炎 黄板树根 白细胞介素-18 滑膜细胞 细胞因子 |
| 英文关键词: Osteoarthritis Huang Ban tree Interleukin 18 Synoviocyte Cytokine |
| 基金项目:国家自然科学基金资助项目（编号30571890） |
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| 中文摘要: |
| 目的：探讨滑膜细胞体外培养方法，了解黄板树根在骨性关节炎（osteoarthritis, OA）中的作用及意义。 方法：取OA患者（n=22）关节滑膜组织，随机分为对照组（n=11）和干预组（n=11），分别原代培养滑膜细胞，采用酶联免疫吸附测定法（ELISA）定量检测两组滑膜细胞培养上清液中炎性因子IL -18蛋白的表达水平。结果：酶消化加组织块贴壁培养法可适用于体外培养试验用滑膜细胞；ELISA检测OA患者对照组细胞培养上清液中IL-18的含量为（61.324 ±19.281） pg/mL，干预组细胞培养上清液中IL-18的含量为（20.823±2.746） pg/mL，具备组间统计学差异（P <0.05）。 结论：黄板树根可以使OA关节滑膜细胞中炎性因子IL-18表达下调，提示其可能对OA有治疗作用。 |
| 英文摘要: |
| Objective:To explore the culture method in vitro about synoviocyte, to study the effect and significance of Huang Ban tree in osteoarthritis.Methods:Gathered the synovium from OA patients（n=22）,divided control group（n=11） and intervention group（n=11）,then take primary cell culture. The concentrations of IL-18 were measured in human OA synoviocyte culture fluid of two group by enzyme-linked immunosorbent assay(ELISA).Results: Enzymatic digestion and tissue culture were refer to synoviocyte culture in vitro.The average value of IL-18 in control group was(61.324 ±19.281) pg/mL.The average value in intervention group was (20.823 ±2.746) pg/mL. There was a significant statistics difference between them（P<0.05）.Conclusion:Huang Ban tree could decrease the expression of IL-18 in synoviocyte of OA.It may play an important therapeutical effect role in OA. |
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