| 张爱娟 张爱元 王赛 季祥武.吡格列酮对实验性兔主动脉粥样硬化的预防作用[J].实用中西医结合临床,2011,(6):1-3 |
| 吡格列酮对实验性兔主动脉粥样硬化的预防作用 |
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| DOI： |
| 中文关键词: 吡格列酮 噻唑烷二酮 动脉粥样硬化 |
| 英文关键词: Pioglitazone Thiazolidinedions Atherosclerosis |
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| 中文摘要: |
| 目的：探讨预防性应用吡格列酮（Piog）对血脂和动脉粥样硬化（AS）斑块的影响。方法： 24只新西兰白兔随机分为四组，I组：正常对照组6只，II组：高胆固醇饮食（Hi-chol）组6只，III组：Hi-chol加高剂量Piog组6只,IV组：Hi-chol加低剂量Piog组6只，喂养8周后处死。于实验终点取兔血清测定血脂；取兔主动脉，观察血管AS斑块及脂质条纹形成情况；免疫组化法测定主动脉血管内皮金属蛋白酶-9（MMP-9）。结果：（1）血脂：Hi-chol成功诱发高脂血症（高TC、TG、LDL-C，HDL-C下降未达显著水平），预防应用高、低剂量Piog明显降低TG，P <0.001。（2）主动脉AS程度：Hi-chol组AS最明显，高、低剂量Piog明显改善主动脉AS情况。（3）主动脉弓血管内壁免疫组化：Hi-chol组、Hi-chol加高低剂量Piog组MMP-9阳性面积比（阳性染色面积/斑块总面积）与正常对照组比较明显升高（P <0.001），高剂量Piog组与单纯Hi-chol组比较，明显降低其表达（P <0.05）。结论：在新西兰兔中，高脂饮食成功诱发高脂血症和动脉粥样硬化，而预防性服用吡格列酮可一定程度上降低TG水平，抑制粥样斑块形成，高剂量吡格列酮降低主动脉粥样斑块内MMP-9表达。 |
| 英文摘要: |
| Objective:To observe the preventive effects of pioglitazone on experiental atherosclerotic rabbits. Methods:24 Healthy WHHL(Watanabe Heritable Hyperlipidemic) rabbits were randomly divided into 4 groups(n=6):I normal control group, II high-cholesterol diet (Hi-chol) group, III hi-chol plus high dose pioglitazone (piog) group, IV Hi-chol plus low dose piog group.All animals were feeding for 8 weeks and sacrificed, taking rabbits blood to measure lipids.Observe rabbit arterial atherosclerosis (AS). Expression of metalloproteinase-9 (MMP-9) in arterial endothelium were detected by immunohistochemistry.Results:（1）Biochemistry:Hi-chol diet successfully induced hyperlipidemia(high TC,TG,LDL-C) compared with normal diet group (P <0.001).High dose piog decreased TG significantly compared with hi-chol diet group (P <0.001).（2）Arterial atherosclerosis: The atherosclerosis in hi-chol diet group was serious than control group. High and low dose piog prevented AS formation significantly.（3）Immunohistochemical staining: High cholesterol diet significantly increased MMP-9 expression of arterial wall(P <0.001), and hi-chol plus hi-dose piog significantly decreased expression of MMP-9 positive area ratio(P<0.05).Conclusions:High cholesterol diet induced hypercholesmia and atherosclerosis in WHHL rabbits. Preventive taking pioglitazone can reduce the elevated TG level, inhibit atherosclerotic plaque formation, decrease the aortic wall MMP-9 expression. |
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