| Objective:To investigate the protective effects of JieDuTongLuoBaoShen Capsules(JC) against renal lesion in diabetic rats.Method:The experimental animals were divided into normal control group,diabetic control group,posivite control group and experimental group.The level of the blood sugar,urea nitrogen blood creatinine,angiotensin II(AngII)were measured and the excretion rate(UAER) of urinary albumin and index of renal hypertrophy were detected in each group at the 12th week after the experiment.At the same time,RT-PCR was used to detect the mRNA expression of renal monocyte chemoattractant protein-1(MCP-1),and immunohistoche mical techniques were used to detect the expressions of renal fibronectin(FN),type IV collagen (Col IV), proliferating cell nuclear antigen(PCNA), nuclear factor Kappa B(NF-?资B),MCP-1, macrophage mark antigen(ED-1).Results:In the JC-treated group,the blood sugar level was markly lowered (P<0.01),UAER was markedly reduced(P<0.05)，the levels of urea nitrogen and blood creatinine were decreased,and the renal hypertrophy index was improved(P <0.01),as compared with those in the diabetic control group.The RT-PCR detection showed that the mRNA expression of renal MCP-1 was increased (P <0.05),and the sediment of glomerular FN 、Col IV、PCNA、NF-?资B、MCP-1 and ED-1were lower than those in diabetic control group (P <0.01).Conclusion:The mechanism of the protective effects of JC against renal lesion may be decreasing the levels of AngII in the renal tissue ,downregulating the level of NF-kB,reducing the expression of MCP-1,and inhibiting the inflammatory pathogenesis of diabetic nephropathy. |
