钟瑜 常克 杨岑 杨国汉.鱼腥草注射液对膜性肾病大鼠的初步实验研究[J].实用中西医结合临床,2005,(1):1-2,32 |
鱼腥草注射液对膜性肾病大鼠的初步实验研究 |
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DOI: |
中文关键词: 膜性肾病 鱼腥草注射液 实验研究 尿蛋白定量 光镜 免疫组化染色 |
英文关键词: houttuynia membranous nephritis Experiment study urinary protein content immunohistochemistry staining |
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中文摘要: |
目的:探讨鱼腥草注射液对膜性肾病大鼠的影响及作用机制。方法:将造模大鼠分为4组:即模型对照组、鱼腥草注射液20mL/Kg组、鱼腥草注射液10mL/Kg组、地塞米松磷酸钠注射液0.1mg/Kg组,连同原有正常对照组,共5个组。实验结束时测定24h尿蛋白定量,并作光镜下观察及免疫组化染色。结果:鱼腥草注射液大、小剂量组24h尿蛋白定量较模型对照组显著减少(P< 0.05);光镜下鱼腥草大剂量组较模型组肾小球周围炎性反应明显减轻,血管内仅有少量红细胞,体视学统计鱼腥草大小剂量组分叶肾小球较模型组有显著性减少(P<0.05),TGF-β1免疫组化染色结果鱼腥草大剂量组肾小球及周围组织着色明显较浅,免疫反应明显减轻。结论:鱼腥草注射液能降低造模大鼠的24h尿蛋白定量,对造模大鼠有减轻其病理损伤的作用。 |
英文摘要: |
Objective:The study was designed to observe the effect of herba houttuyniae on SD MN rats and discuss it's mechanism of action. Methods: SD rats were made to membranous nephritis model by cation bovine serum albumin and divided into 4 groups, the model group, the high-dose and low-dose houttuynia group, Dex group. Heathy rats were used as normal contral group. Twenty-four hours' urinary protein content and plasma albumin, blood lipid were tested by the end of experiment. Pathological changes were observed by using light and used immunohistochemistry staining test. Resutts: As compared to model group, high-dose and low-dose houttuynia group rats showed less urinary protein excretion(P<0.05), Light 、 immunohistochemistry staining all showed that the pathologic changes in the high-dose and low-dose houttuynia group were lesser than that of the model group. Especially the high-dose houttuynia group. Conclusion: Houttuyniae can reduce urinary protein content.Houttuyniae also can reduce the pathologic changes of SD rats were made to membranous nephritis model by cation bovine serum albumin. |
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