| 王南斗 袁作彪 王凌.法尼醇类受体基因在胆囊中的表达[J].实用中西医结合临床,2004,(6):4-5 |
| 法尼醇类受体基因在胆囊中的表达 |
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| DOI： |
| 中文关键词: 法尼醇类受体 胆囊 基因表达 胆结石 |
| 英文关键词: farnesoid X receptor gallbladder gene expression |
| 基金项目:国家自然科学基金资助项目（编号：30271272）及国家基因组南方中心基金资助项目（编号：CNCS-99M-06） |
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| 中文摘要: |
| 目的：探讨法尼醇类受体基因（FXR）的表达与胆囊的关系。方法：采用含17000cDNA克隆的基因微矩阵，与2例正常胆囊之cDNA杂交，发现FXR基因信号后，进一步进行RT-PCR 和测序验证。结果：基因芯片能够检测出FXR在正常胆囊中的表达，其平均灰度值为16.80。在进一步的RT-PCR实验中，扩增出FXR的特异性片段，为352碱基。该PCR片段经测序后，其结果在GENEBANK数据库经BLAST比对，证实其同源性与FXR一致。结论：FXR在胆囊中表达，提示该基因可能还有新的未知功能。 |
| 英文摘要: |
| Objective：To investigate the relationship between the expression of farnesoid X receptor (FXR) gene and gallbladder. Method：cDNA of 2 normal gallbladder tissues was used to hybridize with a cDNA array representing 17,000 cDNA clusters. If the signal of FXR was found, it would be confirmed by reverse transcript polymerase chain reaction (RT-PCR) and sequencing. Results：The signal of FXR, which was 16.80 of gray level, was found in the cDNA array. Then, FXR gene was amplified by RT-PCR, and its peculiar fragment of 352 bp was detected. Furthermore, the sequence of the fragment is confirmed as FXR when it was BLASTed in GENEBANK database.Conclusion：FXR gene is expressed in gallbladder, which suggests a novel function of this gene. |
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