| 吴元胜 范瑞强 禤国维 江光明 孙静.核酸荧光定量PCR检测“疣毒净”洗剂对体外人乳头
瘤病毒清除作用的实验研究[J].实用中西医结合临床,2003,(2):1-2 |
| 核酸荧光定量PCR检测“疣毒净”洗剂对体外人乳头
瘤病毒清除作用的实验研究 |
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| 中文关键词: 尖锐湿疣 HPV “疣毒净” FQ-PCR |
| 英文关键词: point condyloma HPV Verrucous Abluent FQ-PCR |
| 基金项目:广东省自然科学基金“中药‘疣毒净’抗尖锐湿疣复发机理的研究”资助项目。 |
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| 中文摘要: |
| 目的：观察中药“疣毒净”洗液在体外对HPV的影响，以阐明其治疗机理。方法：从临床切除的CA标本中提取HPV，加入中药“疣毒净”后，提取HPV-DNA，用FQ-PCR法测定其病毒含量。结果：含HPV的试管加入“疣毒净”后，经3次反复测试，24h即不能测出HPV，而对照管及非相关中药管均含同等浓度的HPV-DNA拷贝。结论:“疣毒净”具有明确的杀灭HPV的作用，这可能是它治疗CA的机制。 |
| 英文摘要: |
| Purpose: Observation of the influence of Chinese medicine " Verrucous Abluent " liquid to HPV in vitro, in order to expose the curing mechanism of it .Method:After drawing HPV from the CA sample cut off in clinic and adding Chinese medicine " Verrucous Abluent " liquid into the HPV, draw out HPV-DNA and measure its virus content by FQ-PCR . Result:By 3 times measuring repeatedly , HPV could not be surveyed out in 24 hours in the testing tube of " Verrucous Abluent " liquid while contrast tube and non-correlative Chinese medicine tube both had equal concentration of HPV-DNA copy . Conclusion:" Verrucous Abluent " liquid has exact action of killing HPV, which perhaps is its mechanism of curing CA. |
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