基于网络药理学方法研究大黄牡丹汤治疗阑尾炎的作用机制及分子基础

朱远贵; 朱志成; 冯波; 夏雨

文章摘要

朱远贵，朱志成，冯波，夏雨.基于网络药理学方法研究大黄牡丹汤治疗阑尾炎的作用机制及分子基础[J].实用中西医结合临床,2022,22(1):7-10,63

基于网络药理学方法研究大黄牡丹汤治疗阑尾炎的作用机制及分子基础

The Mechanism and Molecular Basis of Rhubarb Peony Decoction Based on Network Pharmacology to Treat Appendicitis

DOI：

中文关键词: 阑尾炎大黄牡丹汤网络药理学作用机制

英文关键词: Appendicitis Rhubarb peony decoction Network pharmacology Mechanism

基金项目:

作者	单位
朱远贵，朱志成，冯波，夏雨	江西省中西医结合医院普外一科

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中文摘要:

目的：运用网络药理学方法研究大黄牡丹汤治疗阑尾炎的作用机制及分子基础。方法：在中药系统药理学数据库及分析平台（TCMSP）中，检索大黄牡丹汤中五味中药的主要化学成分，通过TCMSP平台查找及预测中药化合物对应靶点，在Genecards数据库中搜索阑尾炎疾病靶点，取两者靶点交集导入Swiss平台获得PPI网络，筛选核心作用靶点并构建大黄牡丹汤-潜在靶点-阑尾炎作用网络。对核心靶点进行基因本体（GO）分子功能及基因组百科全书（KEGG）富集分析。结果：筛选得大黄牡丹汤中主要活性成分11个，该活性成分的有效靶点166个，阑尾炎疾病靶点709个，取交集得核心靶点66个；GO富集分析显示靶点基因主要涉及RNA聚合酶Ⅱ启动子的转录正调控、一氧化氮生物合成过程的正调控、平滑肌细胞增殖正调控、蛋白磷酸化正调控等；KEGG富集结果显示大黄牡丹汤可能具有调控FoxO信号通路、鞘脂类信号通路、Toll样受体信号通路等功效。结论：大黄牡丹汤可通过调控机体炎症反应、FoxO信号通路、鞘脂类信号通路、癌症通路、肿瘤坏死因子信号通路，参与促炎症细胞因子的转录调节等治疗阑尾炎。

英文摘要:

Objective: To study the mechanism and molecular basis of rhubarb peony decoction in the treatment of appendicitis by network pharmacology method. Methods: The main chemical components of five traditional chinese medicines in rhubarb peony decoction were searched from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The corresponding targets of TCM compounds were searched and predicted by the TCMSP platform. The appendicitis disease targets were searched in the Genecards database. The intersection of both targets was imported into the Swiss platform to obtain the PPI network. The core targets were screened and the network of rhubarb peony decoction-potential target-appendicitis was constructed. The core targets were enrichment analysis by gene ontology (GO) molecular function and Kyoto Encyclopedia of Genes and Genomes (KEGG). Results: There were 11 main active ingredients in rhubarb peony decoction, 166 effective targets of the active ingredients, 709 targets for appendicitis diseases, 66 intersection core targets; GO enrichment analysis showed that the target genes were mainly involved in positive transcription regulation of RNA polymerase Ⅱ promoter, positive regulation of the nitric oxide biosynthesis, positive regulation of the smooth muscle cell proliferation, positive regulation of the protein phosphorylation, etc. KEGG enrichment results showed that rhubarb peony decoction may have the efficacy of regulating the FoxO signaling pathway, sphingolipid signaling pathway and Toll-like receptor signaling pathway. Conclusion: Rhubarb peony decoction can regulate the body's inflammatory response, FoxO signaling pathway, sphingolipid signaling pathway, cancer pathway, tumor necrosis factor signaling pathway, and participate in the transcription regulation of pro-inflammatory cytokines in the treatment of appendicitis.

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